biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact usFlyers

Technical Notes
View our tech notes
Follow us...
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive

Rabbit antibody to CD133/PROM1: Affinity Purified


Catalogue No. R-1824-100
Description May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). Ref:
Batch No. See product label.
Unit size 100 ug
Antigen E-coli derived human CD133/PROM1 recombinant protein (position: P531-H865) has been used as the immunogen. Human CD133 shares 61% amino acid sequence identity with mouse CD133.
Antibody Type Polyclonal
Isotype IgG
Other Names Prominin-1, Prominin-like protein 1, PROM1, PROML1, Antigen AC133, MSTP061
Accession O43490 PROM1_HUMAN
Produced in Rabbit
Purity Affinity purified
Applications Western Blot (0.1-0.5 ug/mL): tested in MCF-7 whole cell lysate. Immunohistochemistry: paraffin-embedded section (0.5-1.0 ug/mL): tested in human tonsil tissue. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antibody is specific for CD133 as demonstrated by western blotting.
Species Against Human
Antibody Against CD133/PROM1
Cross-reactivity Human, other species not tested yet.
Form Lyophilized.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Rabbit antibody to CD133/PROM1: Affinity Purified A - Western Blot analysis of CD133 expression in MCF7 whole cell lysate (40 ug/lane) at 0.5 ug/mL primary antibody concentration. A band at 130 kDa is observed, consistent with the expected molecular weight of glycosylated CD133/PROM1. B - Immunohistochemistry analysis of CD133 expression in human tonsil tissue.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet


Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
0 items
Email This Page
Tell someone you know about this product.
We Accept PayPal
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment

Official PayPal Seal