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Rabbit antibody to EGFR: affinity purified


Catalogue No. R-1826-100
Description Epidermal Growth Factor (EGF) Receptor binds to EGF and other members of the EGF family such as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. At least 4 isoforms are produced by alternative splicing (reference SWISSPROT). The EGF receptor is a single-pass type I membrane protein containing an extracellular receptor domain, a transmembrane region and an intracellular domain with tyrosine kinase function
Batch No. See product label
Unit size 100 ug
Antigen E.coli-derived human EGFR recombinant protein (Position: L25-K346) was used as the immunogen. Human EGFR shares 89% amino acid (aa) sequence identity with mouse EGFR.
Antibody Type Polyclonal
Other Names Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB;
Accession P00533 EGFR_HUMAN
Produced in Rabbit
Purity Affinity purified
Applications Western Blot (0.1-0.5 ug/mL): tested on human and rat cell lines. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antibody is specific for EGFR as demonstrated by western blotting.
Species Against Human
Antibody Against EGFR
Cross-reactivity Human, rat
Form Lyophilized
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Rabbit antibody to EGFR: affinity purified Western Blot analysis of EGFR expression in HELA (1), A549 (2) and A431 (3) whole cell lysates (40 ug/lane). The observed molecular weight of ~200 kDa corresponds to glycosylated EGFR.

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