||Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
||See product label
||A synthetic peptide (PSESTSHRWRGPA) of human capsaicin receptor protein (aa: 608-621) conjugated to KLH has been used as the antigen.
||Transient receptor potential cation channel subfamily V member 1; TrpV1; Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1; TRPV1; VR1; VR-1;
||IHC. Use at 1:1000 to 1:2000 dilution. This antibody has not been tested in other applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||Specificity was confirmed by IHC using frozen sections of rat dorsal root ganglia (DRG) and spinal cord. Due to sequence homology, similar staining is predicted in human DRG and spinal cord.
||Human, rat. Other species have not yet been tested.
||If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please chose carefully, as the sequence you chose may be a precursor rather than the mature protein for example.
||Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
||After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
||12 months after purchase
||1. Jabin Fagelskiöld A et al (2012) Insulin-secreting INS-1E cells express functional TRPV1 channels.
Islets. 2012 Jan 1;4(1).
||1. Caterina et al. Nature 398: 436(1999).
2. Tominaga et al. Neuron. 21: 531-543(1998).
3. Nagy, I. et al. Eur J Pharmacol 500:351-369(2004).