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Rabbit antibody to human nestin: whole serum


Catalogue No. R-1811-100
Description Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). Ref:
Batch No. See product label.
Unit size 100 uL
Antigen Part of recombinant human protein (amino acids 315-630).
Accession P48681 (NEST_HUMAN)
Produced in Rabbit
Purity Whole serum
Applications Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Reacts with human. Other species not tested.
Form Lyophilised, without preservatives.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Rabbit antibody to human nestin: whole serum Left: SH-SY5Y neuroblastoma cells stained with rabbit antibody to Nestin (red, 1:5,000) and chicken antibody to Vimentin (C-1409-50, green, 1:10,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in SH-SY5Y cell lysate. The antibody (1:2,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.

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