| Catalogue No. |
R-155-100 |
| Description |
MAP1LC3B is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. MAP1LC3B is probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBCELLULAR LOCATION: LC3-I: Cytoplasm. LC3-II: Intracytoplasmic membrane; lipid-anchor. Cytoplasmic vesicle; autophagosome; autophagosomal membrane; lipid-anchor. LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. SIMILARITY: Belongs to the MAP1 LC3 family. |
| Batch No. |
See product label |
| Unit size |
100 uL |
| Antigen |
A synthetic peptide (FEQRVEDVRLIC) corresponding to the N-terminal of human MAP1LC3 B protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from mouse and rat MAP1LC3 B protein. |
| Other Names |
Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated protein 1 light chain 3 beta; MAP1A/MAP1B LC3 B; MAP1A/1B light chain 3 B; MAP1 light chain 3-like protein 2; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; APG8b; MAP-1LC3B; MAP1LC3B; MAP1ALC3; |
| Accession |
MLP3B_HUMAN
MLP3B_MOUSE
MLP3B_RAT |
| Produced in |
Rabbit |
| Purity |
Whole serum |
| Applications |
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
IHC, WB and ELISA confirmed the specificity for MAP1LC3 B. |
| Cross-reactivity |
Human, rat. Other species not yet tested. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
12 months after purchase |
| Specific References |
Theron AE et al. (2013) Molecular crosstalk between apoptosis and autophagy induced by a novel 2-methoxyestradiol analogue in cervical adenocarcinoma cells.
Cancer Cell Int. 2013 Aug 27;13(1):87.
Pilchowski R. et al. (2011) Specific Protein Patterns Characterize Metastatic Potential of Advanced Bladder Cancer.
J Urol. 2011 Jun 16. |
| General References |
1. Difilippantonio S, et al. Eur. J. Cancer 39:1936-1947(2003).
2. Yang Y.-S, et al. Endocr. Relat. Cancer 10:621-627(2003).
3. He H, et al. J. Biol. Chem. 278:29278-29287(2003).
4. Ota T, et al. Nat. Genet. 36:40-45(2004).
5. Tanida I, et al. J. Biol. Chem. 279:36268-36276(2004).
6. Tanida I, et al. J. Biol. Chem. 279:47704-47710(2004).
7. Tanida I, et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004).
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