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Rabbit antibody to Microtubule-associated protein tau (240-450)/MAPT/TAU: IgG


Catalogue No. R-177-250
This product is no longer available. Please see below for our recommended alternative products.
Batch No. See product label
Unit size 250 g
Antigen Recombinant human Microtubule-associated protein tau (aa 240-450) has been used as the immunogen.
Other Names Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; MAPT; MTBT1; TAU
Accession TAU_HUMAN
Produced in Rabbit
Purity Protein G purified IgG
Applications IH/IH(P): This antibody works superbly in both in paraffin-embedded and frozen sections. A working dilution of 1:100 to 1:1000 is recommended. This antiserum recognises both phosphorylated and unphosphorylated forms of Tau protein. Western blot: 1:500-1:2000. Will detect multiple isoforms of Tau; Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity for Tau protein was confirmed by IHC and WB and ELISA.
Cross-reactivity This antiserum cross reacts with Tau protein of human, rat and mouse origins.
Blast it If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised
Reconstitution Reconstitute in 250 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
References 1. Goedert M, et al. (1988) Proc. Natl. Acad. Sci. U.S.A. 85:4051-4055.
2. Goedert M, et al. (1989) Neuron 3:519-526.
3. Lee G, et al. (1989) Neuron 2:1615-1624.
4. Andreadis A, et al. (1992) Biochemistry 31:10626-10633.
5. Jakes R, et al. (1991) EMBO J. 10:2725-2729.
6. Cripps D, et al. (2006) J. Biol. Chem. 281:10825-10838.
7. Maas T, et al. (2000) J. Biol. Chem. 275:15733-15740.
8. Nacharaju P, et al. (1997) J. Neurochem. 69:1709-1719.
9. Wintjens R, et al. (2001) J. Biol. Chem. 276:25150-25156.
10. Oliva R, et al. (2004) Ann. Neurol. 55:448-449.
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