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Rabbit antibody to mouse laminin-111: affinity purified


Catalogue No. R-1808-100
Description Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organisation of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ref:
Batch No. See product label.
Unit size 100 ug
Antigen Laminin-111 isolated from mouse EHS cells
Accession P19137 (LAMA1_MOUSE)
P02469 (LAMB1_MOUSE)
P02468 (LAMC1_MOUSE)
Produced in Rabbit
Purity Affinity purified
Applications Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Reacts with mouse and rat, other species not tested. This antibody recognizes laminin isotypes alpha-1 (440 kDa), beta-1 (220 kDa) and gamma-1 (220 kDa). It also binds laminin binding protein at 120 kDa which always co-expresses with laminin.
Form Lyophilised from a solution containing PBS buffer pH 7.2-7.6 with 5 mM sodium azide.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile water. Centrifuge to remove any insoluble material. Final buffer contains sodium azide as preservative.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Rabbit antibody to mouse laminin-111: affinity purified Left: Staining of laminin in mouse section of cortex by Immunohistochemistry. Rabbit antibody to Laminin (red) reveals strong staining in the basement membranes of blood vessels. Blue: DAPI nuclear stain. Right: Western blot analysis of rat heart cell lysate (Lane 1) and 0.2 ug of purified laminin-111 protein from mouse EHS sarcoma (Lane 2). This antibody recognizes 3 laminin isotypes: alpha-1 (440 kDa), beta-1 (220 kD) and gamma-1 (220 kDa). Also recognized is a laminin-binding protein at 120 kDa in both rat heart lysate and purified laminin protein. Since this protein always co-expresses with laminin this crossreactivity is irrelevant.
Rabbit antibody to mouse laminin-111: affinity purified Left: Analysis of rat brain stem section stained with rabbit anti-laminin (red, 1:1,000), and chicken anti-myelin basic protein (MBP, C-1383-50, green, 1:5,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained.The laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons. Right: Western blot analysis of laminin expression (green, 1:5,000). [2] rat brain, [3] rat spinal cord, and [4] cow spinal cord. The strong band above the 250 kDa mark corresponds to full-length laminin protein. Smaller proteolytic fragments of laminin are also detected with this antibody.
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