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Rabbit antibody to mouse TROY (75-88): whole serum


Catalogue No. R-091-100
Description FUNCTION: Can mediate activation of c-Jun and NF-kappa-B. May promote caspase-independent cell death. Isoform 2 and isoform 3 may act as decoy receptors. SUBUNIT: Associates with TRAF1, TRAF2, TRAF3 and TRAF5. SUBCELLULAR LOCATION: Isoform 1, isoform 3, isoform 4: Cell membrane; single-pass type I membrane protein (Probable). Isoform 2: Secreted protein (Probable). ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Highly expressed in adult brain, and in embryos from day 11-17, but not earlier. Detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In neonatal mice, mainly in hair follicles and neuron-like cells in the cerebellum, but not in the skin epidermis. Isoform 3 was found in embryonic day 17.5 skin but not in brain and liver. SIMILARITY: Contains 3 TNFR-Cys repeats.
Batch No. See product label
Unit size 100 uL
Antigen A synthetic peptide (CRPHRF KEDWGFQK) as part of mouse TROY protein (aa: 75-88) conjugated to the immunogenic protein Blue Carrier Protein
Other Names Tumor necrosis factor receptor superfamily member 19; TNFRSF19; Toxicity and JNK inducer; TRADE
Accession TROY_MOUSE
Produced in Rabbit
Purity Whole serum
Applications IHC. Recommended to be used at a dilution of 1:500 to 1:2000 for immunohistochemistry. This antiserum has not yet been tested for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity for TROY was confirmed by IHC.
Cross-reactivity This antiserum is known to react with rat TROY. Reactivity with other species have not yet been tested.
Blast it If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
References 1. Hisaoka, T. (2004). Glia 45:313-324.
2. Hisaoka, T. (2006b). Eur J Neurosci 23:3149-3160.
3. Kojima, T. (2000). J Biol Chem 275:20742-20747.
Images (click to zoom)
Rabbit antibody to mouse TROY (75-88): whole serum The cryostat section of trigeminal ganglia was stained with the Rabbit antibody to mouse TROY (75-88): whole serum at the dilution of 1: 2000. Following incubation of the section with the primary antibody overnight and secondary antibody for 2 hours, the section was developed with diaminobenzidine substrate with nickel sulfate enhancement.

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