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Rabbit antibody to Tyrosine Hydroxylase (TH): IgG


Catalogue No. R-1645-500
Description Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Related products R-118-100. R-1645-500 is the Protein G-purified version of R-118-100 (whole serum).
Batch No. See product label
Unit size 500 ug
Antigen A synthetic peptide (PRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (63-78) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (31-47).
Other Names TH; Tyrosine hydroxylase; Tyrosine 3-monooxygenase; L-tyrosine hydroxylase; Tyrosine 3-hydroxylase
Accession P04177 TY3H_RAT;
Produced in Rabbit
Purity Protein G purified
Applications IHC. A concentration of 4-10 ug/mL is recommended for this application. This is a superb antibody for detection of tyrosine hydroxylase containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals. We recommend mouse or rat brain containing catecholaminergic neurons as a positive control for this antibody, for example brain stem or striatum. Western blotting: A concentration of 5-15 ug/mL is recommended.Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity IHC on brain shows a pattern of staining specific for TH containing neurons.
Cross-reactivity This antibody is known to react with rat, mouse and guinea pig. Cross reactivity with other species has not yet been tested.
Blast it If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised from PBS, pH 7.4, without preservatives.
Appearance White powder
Reconstitution Reconstitute in 500 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Specific References 1. Pierre S.R., Lemmens M.A., Figueiredo-Pereira M.E. (2009) Subchronic infusion of the product of inflammation prostaglandin J2 models sporadic Parkinson's disease in mice J Neuroinflammation. Jul 25;6:18
2. Takeoka A. et al (2010) Noradrenergic innervation of the rat spinal cord caudal to a complete spinal cord transection: effects of olfactory ensheathing glia J Exp Neurol. 2010 Mar;222(1):59-69.
3. Brown R.E. et al (2008) Characterization of GABAergic neurons in rapid-eye-movement sleep controlling regions of the brainstem reticular formation in GAD67-green fluorescent protein knock-in mice. Eur J Neurosci. 2008 Jan;27(2):352-63.
4. Bisem NJ et al (2012) Mapping of FGF1 in the Medulla Oblongata of Macaca fascicularis. Acta Histochem Cytochem. 2012 Dec 26;45(6):325-34.
References 1. Mallett, J. Trends in Pharmacological Science. 17(4): 129-135, 1996.
2. Haavik, J. et al. Mol. Neurobiology 16(3) :285-309, 1998.
3. Lewis DA, et al, Neuroscience 54: 477, 1993
4. Kumer S.C. et al. Journal of Neurochemistry, 67(2) :443-462, 1996.
5. Haycock, J. Anal. Biochemistry 181: 259-266, 1989.
6. Haycock, J. Anal. Biochemistry 208: 397-399, 1993.
7. Renfroe, J.B., et al. Brain Res. Bull. 13: 109-126, 1984.
8. Xu, Z et a.l Neurosci. 82(3): 727, 1998
Images (click to zoom)
Rabbit antibody to Tyrosine Hydroxylase (TH): IgG Immunohistochemical staining of catecholaminergic neurons in the rat brain stem.
Rabbit antibody to Tyrosine Hydroxylase (TH): IgG Immunohistochemical staining of catecholaminergic neurons in the rat brain stem.
Rabbit antibody to Tyrosine Hydroxylase (TH): IgG Separation and detection of tyrosine hydroxylase in PC12 cell lysates (6 μg protein/lane) by SDS-PAGE (reduced) and western blotting with anti-tyrosine hydroxylase antibody (1:100 dilution of R-118-100). Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa. MWM: molecular weight marker
Rabbit antibody to Tyrosine Hydroxylase (TH): IgG Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with the rabbit polyclonal TH antiserum (R-118-100; 1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
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