| Catalogue No. |
R-079-100 |
| Description |
TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats. |
| Batch No. |
See product label |
| Unit size |
100 uL |
| Antigen |
A synthetic peptide (C-KNSASEEDHLPLQVLQSP) of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen. |
| Other Names |
osm-9-like TRP channel 2; OTRPC2 |
| Accession |
VRl_RAT |
| Produced in |
Rabbit |
| Purity |
Whole serum |
| Applications |
IHC, Immunofluorescence, Western blot. Recommended to be used at a dilution of 1: 1000 to 1: 2000 for immunohistochemistry and Western blot, for Immunofluorescence at a dilution of 1:50 to 1: 200 in free-floating sections or paraffin embedded sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
Immunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot. |
| Cross-reactivity |
This antibody is known to react with rat and human VRL-1. |
| Blast it |
If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. |
| Expiry Date |
12 months after purchase |
| References |
1. Caterina, et al., Nature 398, 436-41 (Apr 1, 1999).
2. Jahnel, et al., Eur J Biochem 270, 4264-71 (Nov, 2003).
3. Montell, et al., Mol Cell 9, 229-31 (Feb, 2002). |
Images (click to zoom)
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Immunofluorescence staining of human brain (midfrontal cortex, Alzheimer case) fixed in Zamboni. Dilution used: 1:50 (catalogue # R-079-100), free-floating sections, 40X magnification. |
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The rat spinal cord section was stained with Rabbit antibody to VRL-1 (744-761): whole serum at a dilution of 1: 1000. Nerve terminals in the dorsal horn are labelled. Medium to large sized sensory neurons in the dorsal root ganglia are labelled by this antiserum. |
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