| Catalogue No. |
R-1682-500 |
| Description |
Autosomal recessive mutations in DJ-1 cause early-onset familial Parkinson's disease. DJ-1 is considered a redox-sensitive cytoplasmic protein found in brain as well as other cell types. |
| Batch No. |
See product label |
| Unit size |
500 ug |
| Antigen |
A synthetic peptide (ASKRALVILAKGAEE-C) corresponding to human DJ-1 [2-16] in the N-terminal domain conjugated via additional C-terminal Cys to Diphtheria toxoid. |
| Antibody Type |
Polyclonal |
| Other Names |
PARK7 |
| Produced in |
Rabbit |
| Applications |
WB and IHC. Suggested dilution of 1:5,000 is recommended for WB. DJ-1 is a soluble protein of 189 amino acids and detected with 23 kDa mobility by western blotting. The suggested dilution for IHC is 1:100. Detected astrocyte cytoplasmic labelling in human brain formaldehyde-treated tissue. Biosensis recommends that the optimal working dilution should be determined by the end user. |
| Specificity |
Confirmed by WB using soluble mouse and human brain extracts, reactivity for major product diminished by peptide absorption. |
| Species Against |
Human and mouse DJ-1 are highly conserved, so cross-reactivity with other species is expected. |
| Form |
Lyophilized from PBS, pH 7.4. Contains no preservative. |
| Reconstitution |
Reconstitute in 500 uL of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
Short term storage at 2-8C for one week. At -20C as an undiluted liquid for up to 12 months. |
| Expiry Date |
12 months after purchase |
| General References |
Sonia George, Su San Mok, Milawaty Nurjono, Scott Ayton, David I. Finkelstein, Colin L. Masters,Qiao-Xin Li & Janetta G. Culvenor (2010) alpha-Synuclein Transgenic Mice Reveal Compensatory Increases in Parkinson's Disease-Associated Proteins DJ-1and Parkin and Have Enhanced alpha-Synuclein and PINK1 Levels After Rotenone Treatment J Mol Neurosci 42:243_254 |
