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Rabbit polyclonal antibody to Annexin V (88-102): Affinity purified


Catalogue No. R-1022-100
Description Annexin V, also known as Annexin A5, belongs to the annexin family of calcium dependent phospholipid binding proteins. Annexin V has roles in cellular signal transduction, inflammation, growth and differentiation. Annexin V has been shown to have a high affinity for phosphatidylserine (PS) and is therefore a potential probe for the detection of apoptosis.
Batch No. See product label
Unit size 100 ug
Antigen A synthetic peptide corresponding to a region (88-102 aa) from human Annexin V.
Other Names Annexin A5; Annexin 5; Lipocortin V; Endonexin II; Calphobindin I; PAP-I; PP4; VAC-alpha; Anchorin CII; CBP-I; ANX5; ENX2;
Accession P08758 ANXA5_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 ug/mL is recommended for WB. Human Annexin V has a predicted length of 320 residues and a MW of 36 kDa. A concentration of 0.5-1 ug/mL is recommended to detect Annexin V in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; rat; predicted to react with mouse due to sequence homology;
Blast URL Click here
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal,0.05mg NaN3
Reconstitution Reconstitute in 100 uL of sterile distilled water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase
Specific References Wei X et al. (2016) "Fatty acid synthesis configures the plasma membrane for inflammation in diabetes." Nature. [Epub ahead of print]. Application: WB. Species: Mouse.
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