||Beta-casein has an important role in determination of the surface properties of the casein micelles. it is cleaved into 3 chains (casoparan, casohypotensin and antioxidant peptide). Casoparan acts as a macrophage activator, increasing the phagocytic activity of macrophages and peroxide release from macrophages. It also acts as a bradykinin-potentiating peptide. Casohypotensin acts as a bradykinin-potentiating peptide and induces hypotension in rats. Acts as a strong competitive inhibitor of endo-oligopeptidase A. Antioxidant peptide has antioxidant activity (ref: uniprot.org.).
Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
|| C-1780-100: Chicken antibody to bovine A2 beta casein: Affinity purified.
C-1781-100: Chicken antibody to bovine A1 beta casein: Affinity purified.
||See product label
||Bovine beta-casein peptides
||ELISA and western blotting (1:5,000 - 1: 20,000). Do not use skim milk for blocking and dilution of assay reagents, BSA is recommended (3-5% in blocking and 1-3% in dilution buffer). Biosensis recommends that optimal working dilutions should be determined by the end user.
||Bovine beta-casein. Detects both A1 and A2 beta-casein isoforms.
||Species cross-reactivity not tested.
||Liquid in PBS, pH 7.4 with 0.02% sodium azide. Refer to the product label for antibody concentration.
||Maintain unopened vial at -20degC for up to 12 months after date of receipt. Prior to opening it is recommended that the tube is microfuged for a few seconds to insure that all liquid is in the bottom of the vial. After opening maintain at -20C in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8C for up to one week. Avoid repeated freeze-thaw cycles.
||12 months after date of receipt unopened and stored at -20degC.