||CD22 is a single-pass type 1 membrane glycoprotein found in the cytoplasm and cell membrane of B-lymphocytes. CD22 has at least two isoforms (alpha and beta) produced by alternate splicing. The protein exists predominantly as a monomer of the beta isoform (95 kDa). It is also found as a heterodimer of the beta and alpha isoforms.
||See product label
||A synthetic peptide corresponding to a region (656-670 aa) from human CD22.
||SIGLEC2; Siglec-2; Leu-14; BL-CAM; CD22 antigen;
||Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 ug/mL is recommended for WB. Human CD22 has a predicted length of 847 aa (beta isoform) and a MW of 95 kDa. A concentration of 0.5-1.0 ug/mL is recommended to detect CD22 in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
||Reconstitute in 100 uL of sterile distilled water to achieve an antibody concentration of 1 ug/ul. Centrifuge to remove any insoluble material.
||At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
||12 months after purchase.