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Rabbit polyclonal antibody to CD40 Ligand (42-56): Affinity purified


Catalogue No. R-1014-100
This product is no longer available. Please see below for our recommended alternative products.
Replaced by R-1622-100
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide (FAVYLHRRLDKIEDE) corresponding to amino acid sequence 42-56 from the N-terminus of human CD40L.
Other Names CD40-L; TRAP; gp39; TRAM; CD154; Tumour necrosis factor ligand superfamily member 5; TNFSF5; CD40L;
Accession P29965 CD40L_HUMAN; P27548 CD40L_MOUSE
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 g/ml is recommended for WB. Human CD40L has a predicted length of 261 residues and MW of 29 kDa. The observed size is 32 kDa. A concentration of 1.0 g/ml is recommended to detect CD40L in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; rat; expected to react with mouse due to sequence homology;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please chose carefully, as the sequence you chose may be a precursor rather than the mature protein for example.
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Appearance White powder
Reconstitution Reconstitute in 100 l of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to CD40 Ligand (42-56): Affinity purified Immunohistochemical staining of paraffin-embedded human mammary cancer sections using CD40L antibody (R-1014-100) at a concentration of 1.0-2.0μg/ml.  Secondary detection with Biotinylated goat anti-rabbit IgG (10.0μg/ml).

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