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Rabbit polyclonal antibody to Connexin-43 (366-382): Affinity purified


Catalogue No. R-1607-100
Description A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighbouring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin-43, also known as Gap junction alpha-1 (GJA1), belongs to the class II (alpha) group of connexins and is expressed in the heart and foetal cochlea.
Batch No. See product label
Unit size 100 ug
Antigen A synthetic peptide corresponding to the amino acids 366-382 from the C-terminus of human Connexin-43.
Other Names Cx43; gap junction alpha-1 protein; GJA1; GJAL; Gap junction 43 kDa heart protein;
Accession P17302 CXA1_HUMAN; P23242 CXA1_MOUSE; P08050 CXA1_RAT
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 ug/mL is recommended for WB. The predicted length of human Connexin-43 is 382 residues and MW of 43 kDa. A concentration of 0.5-1.0 ug/mL is recommended to detect Connexin-43 in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Cross-reactivity Human; mouse; rat;
Blast URL Click here
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 uL of sterile distilled water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
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