||Human beta-Lipotropin is a 93 amino acid polypeptide that is cleaved from carboxy-terminal fragment of the precursor pro-opiomelanocortin (POMC). It stimulates melanocytes to produce melanin, and can also be cleaved into smaller peptides including opioid peptides: gamma-lipotropin, alpha-MSH, beta-MSH, gamma-MSH, alpha-endorphin, beta-endorphin, gamma-endorphin and met-enkephalin
||See product label
||Synthetic peptide (179-267 aa) of human beta-Lipotropin conjugated to thyroglobulin was used as the immunogen.
||Beta-LPH; Lipotropin beta; Pro-opiomelanocortin; POMC;
||P01189 COLI_HUMAN; P01190 COLI_BOVIN;
||A dilution of 5-10 ug/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 ug/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||Human; mouse; rat. Beta-Lipotropin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Leu-enkephalin 0.01%; with Met-Enkephalin 0.01%; with beta-endorphin 0.01%
||If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between proteins. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
||Lyophilised with BSA
||Reconstitute in 0.05 mL of PBS (pH 7.4). Centrifuge to remove any insoluble material.
||At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability and at 2-8C with an appropriate antibacterial agent. Avoid freeze-thaw cycles
||12 months after purchase