Heat shock protein beta-1 (HSPB1) belongs to the small heat shock protein (HSP20) family. HSPB1 is expressed in response to heat shock and is also activated by estrogen in hormone responsive cells. HSPB1 is expressed in most cells with highest expression in heart and in tissues composed of striated and smooth muscle.
||See product label
||A synthetic peptide (TIPVTFESRAQLGGP) corresponding to a region (180-194) from human Heat shock protein beta-1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
||Heat shock protein beta-1; HspB1; Heat shock 27; HSP 27; Stress-responsive protein 27; SRP27; Estrogen-regulated 24 kDa protein; 28 kDa heat shock protein; HSPB1; HSP27; HSP28;
||Affinity purified on antigen column
||Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 ug/mL is recommended for WB. Human HSPB1 has a predicted length of 205 residues and MW of 23 kDa. A concentration of 0.5-1.0 ug/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||The specificity of this antibody has been confirmed by WB and IHC against the antigen.
||Human; expected to react with rat and mouse due to sequence homology
||If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
||Liquid (0.5ml). 50% glycerol, 0.9mg NaCl and 0.2mg Na2HPO4
||The liquid formulation should be diluted in PBS (pH 7.4)
||After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
||12 months after purchase