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Rabbit polyclonal antibody to ICAM1 (503-516): Affinity purified


Catalogue No. R-1025-100
Intercellular adhesion molecule 1 (ICAM1) is a single-pass type 1 membrane glycoprotein that belongs to the ICAM family. ICAM1 is typically expressed on endothelial cells and cells of the immune system. ICAM-1 is also expressed in all types of glial cells including astrocytes and microglia.
Batch No. See product label
Unit size 100 ug
Antigen A synthetic peptide (YNRQRKIKKYRLQQ) corresponding to a region (503-516) from the C-terminus of human ICAM1.
Other Names ICAM1; ICAM-1; Intercellular adhesion molecule 1; CD54;
Accession P05362 ICAM1_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 ug/mL is recommended for WB. Human ICAM1 (precursor) has a predicted length of 532 residues and a MW of 58 kDa. A concentration of 0.5-1 ug/mL is recommended to detect ICAM1 in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please chose carefully, as the sequence you chose may be a precursor rather than the mature protein for example.
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 uL of sterile distilled water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase
References 1. Satoh J. et al. Cytokine-induced expression of intercellular adhesion molecule-1 (ICAM-1) in cultured human oligodendrocytes and astrocytes. J Neuropathol Exp Neurol. 1991 May;50(3):215-26.
2. Verbeek M.M. Accumulation of intercellular adhesion molecule-1 in senile plaques in brain tissue of patients with Alzheimer's disease. Am J Pathol. 1994 January; 144(1): 104116.
3. Heppner F.L. et al. Vitamin E induces ramification and downregulation of adhesion molecules in cultured microglial cells. Glia. 1998 Feb;22(2):180-8.
4. Yang Y. et al. Evaled expression of ICAM-1 and its ligands in the rat spinal cord following lipopolysaccharide intraspinal injection. Neuromolecular Med. 2008;10(4):385-92.
Images (click to zoom)
Rabbit polyclonal antibody to ICAM1 (503-516): Affinity purified Immunohistochemical staining of paraffin-embedded human mammary cancer sections using ICAM1 antibody (R-1025-100) at a concentration of 1.0-2.0μg/ml.  Secondary detection with Biotinylated goat anti-rabbit IgG (10μg/ml).

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