| Catalogue No. |
R-1605-500 |
| Description |
Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot). |
| Batch No. |
See product label |
| Unit size |
500 ug |
| Antigen |
A synthetic peptide corresponding to a region (1-20 aa) from the N-terminus of human Presenilin 1 conjugated to Diptheria toxoid. |
| Sequence |
MTELPAPLSYFQNAQMSEDN-C |
| Antibody Type |
Polyclonal |
| Other Names |
Presenilin 1; PS-1; Protein S182; PS1-CTF12; PSEN1; AD3; PS1; PSNL1; |
| Accession |
P49768 PSN1_HUMAN; |
| Produced in |
Rabbit |
| Applications |
IF and WB. Suggested dilution of 1:2,000 is recommended for WB. On SDS-PAGE, the predominant form detected by this antibody is the N-terminal Presenilin 1 fragment of approx 29 kDa. The uncleaved form of Presenilin 1 migrates to approx 45 kDa. Human and mouse brain samples commonly prepared with reducing agent (50mM DTT), urea (2.3M), SDS (1%) in 62.5 mM Tris-HCl pH 6.8 sample buffer heated to 50C for 15 min. The suggested dilution for IF is 1:100 for acetone or paraformaldehyde fixed cells or tissue. Biosensis recommends that the optimal working dilution should be determined by the end user. |
| Specificity |
Specificity confirmed by WB and IF using transfected cells, Presenilin 1 knock-out mouse cells, mouse and human brain. |
| Species Against |
Human; mouse; rat; guinea pig. Expected to cross-react with other species due to sequence homology. |
| Blast URL |
Click here |
| Conjugate |
Diptheria toxoid covalently linked to additional Cys residue using maleimidocaproyl-N-hydroxysuccinimide |
| Form |
Lyophilized from PBS, pH 7.4. Contains no preservative. |
| Reconstitution |
Reconstitute in 500 uL of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
Short term storage at 2-8C for one week. At -20C as an undiluted liquid for up to 12 months. |
| Expiry Date |
12 months after purchase |
| References |
1. Culvenor, J.G., Ilaya, N.T., Ryan, M.T., Canterford, L., Hoke, D., Williamson, N.A., McLean, C.A., Masters, C.L., and 1. Evin, G. (2004) Characterization of Presenilin complex from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with Presenilin mutations. Eur. J. Biochem. 271, 375-385.
2. Ilaya, N.T., Evin, G., Masters, C.L., and Culvenor, J.G. (2004) Nicastrin expression in mouse peripheral tissues is not co-ordinated with Presenilin and is high in muscle. J. Neurochem. 91, 230-237.
3. Beher, D., Fricker, M., Nadin, A., Clarke, E.E., Wrigley, J.D.J., Li, Y.-M., CULVENOR, J.G., Masters, C.L., Harrison, T., and Shearman, M.S. (2003) In vitro Characterization of the Presenilin-dependent γ-secretase complex using a novel affinity ligand. Biochem. 42, 8133-8142.
4. Evin, G., Smith, M.J., Tziotis, A., McLean, C., Canterford, L., Sharples, R.A., Cappai, R., Weidemann, A., Beyreuther, K., Cotton, R.G.H., Masters, C.L., and Culvenor, J.G. (2002) Alternative transcripts of Presenilin-1 associated with Frontotemporal Dementia. NeuroReport 13, 917-921.
5. Silveyra, M.-X., Evin, G., Montenegro, M.-F., Vidal, C.J., Martínez, S., Culvenor, J.G., and Sáez-Valero, J. (2008) Presenilin-1 interacts with acetylcholinesterase and alters its enzymatic activity and glycosylation. Mol. Cell Biol. 210, 788-792. |
Images (click to zoom)
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Western Immunoblotting of human and mouse Presenilin 1 protein in mouse cell line extracts and mouse and human brain. Membrane protein load 20 µg. Crude anti-PS1 NT used at 1:2000. |
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Presenilin 1 detection in PS1 positive and knock-out neurosphere derived cells after differentiation. Immunofluorescence after 4% paraformaldehyde fixation for 15 min, PS1[1-20] protein A-purified (20μg/ml). |
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