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Rabbit polyclonal antibody to Ubiquitin C Terminal Hydrolase 1: Whole serum

$297.00USD


Catalogue No. R-1408-50
Description This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
Batch No. See product label
Unit size 50 uL
Antigen Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
Antibody Type Polyclonal
Other Names Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1;
Accession P09936 UCHL1_HUMAN;
Produced in Rabbit
Purity Antiserum
Applications Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate.
Species Against Human
Antibody Against Ubiquitin C Terminal Hydrolase 1
Cross-reactivity Rat, mouse, horse, cow, pig, chicken
Blast URL Click here
Form Lyophilised
Reconstitution Reconstitute in 50 uL sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to Ubiquitin C Terminal Hydrolase 1: Whole serum Image shows rat mixed neuron/glial cultures stained with Rabbit polyclonal antibody to Ubiquitin C Terminal Hydrolase 1 R-1408-50 (green). Blue is a DNA stain. Note that the Ubiquitin C Terminal Hydrolase 1 (UCHL1) antibody stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also. Surrounding glial cells are not stained with this antibody, though many are present as visualized using the DNA stain.
Rabbit polyclonal antibody to Ubiquitin C Terminal Hydrolase 1: Whole serum Left: Analysis of SH-SY5Y cells for UCHL1 expression with rabbit antibody to UCHL1 (green, 1:1,000) by Immunocytochemistry. Cells were co-stained with mouse antibody to fibrillarin (M-1372-250, red, 1:1,000). Blue: DAPI nuclear stain. The UCHL1 antibody produces strong staining of the cellular cytoplasm of these cells which share many properties with neurons, while the fibrillarin antibody specifically labels nucleoli. Right: Western blot analysis of tissue and cell lysates using rabbit antibody to UCHL1 (red, 1:2,000, lanes 2-7). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24 kDa corresponds to the UCHL1 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties, but not in HeLa, NIH-3T3 and other non-neuronal cells. The blot was simultaneously probed with a mouse antibody to HSP60 (green).
PDF Data Sheet

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MSDS

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