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SARS-CoV-2 S1 Receptor-Binding Domain (S1RBD) IgG ELISA Kit (4 Plates)


Catalogue No. BEK-1002-4P
Description The SARS-CoV-2 S1RBD IgG ELISA kit is a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection and qualitative measurement of IgG class antibodies against the spike protein S1 receptor-binding domain (S1RBD) of SARS-CoV-2 virus (causing Covid-19) in human blood.

The Biosensis SARS-CoV-2 S1RBD IgG ELISA kit is a two-step incubation immunoassay kit. Recombinant spike protein S1 receptor-binding domain (S1RBD) of SARS-CoV-2 pre-coated onto the polystyrene microwell strips can specifically recognize anti-S1RBD antibodies in human serum or plasma specimens. After a 1 hour incubation, anti-S1RBD antibodies are captured by immobilized S1RBD protein while the unbound components are washed away. Afterwards, a detection solution containing HRP-conjugated anti-human IgG is added for another 1 hour incubation, wherein HRP-conjugated anti-human IgG binds to the IgG class antibodies previously bound to S1RBD protein on the plate. After removal of nonspecific bindings, an HRP substrate solution containing 3,3′,5,5′-Tetramethylbenzidine (TMB) is added, resulting in the formation of a blue color. Color reaction is stopped by 2M H2SO4, transforming the blue color to yellow, which is quantified by an absorbance microplate reader at 450 nm. The color intensity is proportional to the amount of anti-S1RBD antibodies captured inside the wells.

This product is intended for use by professional persons only.† For research use only. Not for use in clinical & diagnostic procedures. In line with FDA guidelines, test reports must include the following statements:

(1) This test has not been reviewed by the FDA.
(2) Negative results do not rule out SARS-CoV-2 infection, particularly in those who have been in contact with the virus.
(3) Follow-up testing with a molecular diagnostic should be considered to rule out infection in these individuals.
(4) Results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status.
(5) Positive results may be due to past or present infection with non-SARS-CoV-2 coronavirus strains, such as coronavirus HKU1, NL63, OC43, or 229E
Related products SARS-CoV-2 Nucleocapsid Protein (NP) IgG ELISA Kit
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SARS-CoV-2 Nucleocapsid Protein (NP) Protein
SARS-CoV-2 Spike Protein S1 Receptor-Binding Domain (S1RBD) Protein
Batch No. Refer to the product label
Unit size 4 x 96 wells (12 strips of 8 wells)
Antigen The spike protein (S) is an envelope-anchored protein that mediates the recognition and binding of SARS-CoV-2 to host cells. S can be further cleaved by the host protease into two subunits called S1 and S2. S1 polypeptide contain a receptor binding domain (S1RBD) crucial for the specific recognition and interaction with the human receptor ACE2, which is the first and the most essential step for the virus infection.
Other Names Surface glycoprotein [Severe acute respiratory syndrome coronavirus 2]; S1RBD fragment
Accession GenBank: QHD43416.1
Applications Human blood samples, including serum, EDTA-, sodium citrate- and heparin-plasma.
Specificity Clinical validation study of Biosensis SARS-CoV-2 S1RBD IgG ELISA was conducted in 2020 in Shenzhen, China. Samples were collected from COVID-19 confirmed cases with clinical symptoms, laboratory abnormalities or pulmonary imaging manifestations. No tests have been performed on specimens from latent infections or patients in the incubation period. The kit showed higher positive detection rate in specimens from patients with delayed onset. Therefore, the interpretation of the test results should consider the specimenís collection time. It is highly recommended that each laboratory establishes its own normal and pathological reference range for anti-S1RBD IgG level. Furthermore, it is also recommended that each laboratory includes its own panel of control samples in the assay.
Storage Store at 2-8C.
Expiry Date The unopened kit is stable for 6 months from date of receipt when stored at 2-8C. Once opened, store reagents at 2-8C for up to 1 month.
Kit components The ELISA kit box contains 4 x 96-well pre-coated strip plates, blank control sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Kit protocol Click to download the kit protocolBEK-1002_as_at_April2020.pdf
MSDS Click to download the Material Safety Data SheetBiosensis SARS_CoV-2_ELISA ASSAY MSDS 5-2020.pdf
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SARS-CoV-2 S1 Receptor-Binding Domain (S1RBD) IgG ELISA Kit (4 Plates) Performance Characteristics

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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