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Sheep antibody to Ancient ubiquitous protein 1 (48-60): whole serum


Catalogue No. S-035-100
Description AUP1 contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain thay may be involved in binding ubiquitin-conjugating enzymes. The unprocessed precusor is of 476 amino acids in length and has an estimated molecular weight of 53 kDa. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing.TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the AUP1 family. SIMILARITY: Contains 1 CUE domain.
Batch No. See product label
Unit size 100 uL
Antigen A synthetic peptide (HVFLVSCALPDSV) corresponding to the amino acids 48-60 of human ancient ubiquitous protein 1 conjugated to Blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from bovine, mouse and rat.
Other Names AUP1
Accession AUP1_HUMAN
Produced in Sheep
Purity Whole serum
Applications IHC, WB. Use at a dilution of 1:500 to 1:6000. This antiserum works superbly in both paraffin embedded and frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antiserum is specific for ancient ubiquitous protein 1.
Cross-reactivity This antibody is known to react with bovine, sheep, human and rat ancient ubiquitous protein 1. Other species have not yet been tested.
Blast it If you would like to use this product in another speices other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
References 1. Wilkinson, K.D. et al. J. Biol. Chem. 256, 9235-9241, (1981)
2. Schwartz, A.L. et al. Annu. Rev. Med. 50, 57-74, (1999)
3. Coux, O., et al. Annu. Rev. Biochem. 65, 801-847, (1996)
4. Strausberg R.L, et al. Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
5. Jang W, et al. Genomics 36:366-368(1996).
6. Shibata K, et al. Genome Res. 10:1757-1771(2000).
7. Carninci P, et al. Genome Res. 10:1617-1630(2000).
8. Kawai J, et al. Nature 409:685-690(2001).
9. Okazaki Y, et al. ature 420:563-573(2002).
10. Carninci P, et al. Science 309:1559-1563(2005).
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