||FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
||See product label
||Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
||Beta-nerve growth factor
||Protein G purified IgG
||IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 ug/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 ug/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
||This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
||If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
||Reconstitute in 500 uL of sterile water. Centrifuge to remove any insoluble material.
||Maintain lyophilized material at 2-8degC or -20degC for up to 12 months after date of receipt. After reconstitution keep aliquots at -20degC for up to six months for a higher stability, and at 2-8degC with an appropriate antibacterial agent for up to one week. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
||12 months after purchase (lyophilized)
||Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC; Species: Rat
||1. Ebendal, T. et al (1989) J Neurosci Res 22, 223-240.
2. Zhou, X. F. et al (1994) J Neurosci Methods 54, 95-102.
3. Angeletti, P. U. et al (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-75.
4. Hesse K. et al. (1997) Neurosci Lett. Aug 8;231(2):83-6.
5. Miao J et al. (2012) Neurosci Res. Dec;74(3-4):269-76.