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Sheep antibody to human proNGF: whole serum


Catalogue No. S-080-100
Description Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released. This antibody is raised in sheep to detect the prodomain of NGF not the mature peptide.
Batch No. See product label
Unit size 100 uL
Antigen The recombinant prodomain fragment of human nerve growth factor
Other Names pro-brain nerve growth factor; proNGF; NGF;
Accession NGF_HUMAN
Produced in Sheep
Purity Whole serum
Applications IHC, Immunofluorescence, ELISA, Western Blot, biological neutralization of proNGF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB. It does NOT crossreact with proBDNF, proNT-3 or mature NGF.
Cross-reactivity Confirmed to react with purified human proNGF and crossreact with mouse and rat proNGF
Blast it If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule.
Form Lyophilised
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage Maintain lyophilized antibody at 2-8C for up to 12 months after date of receipt. After reconstitution keep undiluted aliquots at -20C for up to 6 months for higher stability or at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase (lyophilized)
Specific References Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Western Blotting.
General References Srinivasan B et al. J Biol Chem. 2004 Oct 1;279(40):41839-45.
Yune TY et al. J Neurosci. 2007 Jul 18;27(29):7751-61.
Images (click to zoom)
Sheep antibody to human proNGF: whole serum Western blot of proNGF proteins produced in E.Coli. S-080-100 detects the human and mouse form of proNGF as well as the pro-domain peptide. SDS-PAGE: denatured and reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 um); Blocking: 5% skim milk in TBST, 2 hours at RT; Primary antibody: overnight at 4°C (1:1000 dilution); Secondary antibody: anti-sheep-HRP (1/3000), 2 hours at RT; Detection: Chemiluminiscence.
Sheep antibody to human proNGF: whole serum Immunofluorescence analysis of proNGF expression in human DU145 prostate cancer cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) DU145 cells were incubated with proNGF antibody S-080-100 (1:1000, red) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-sheep-PE antibody (1:100, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). Magnification: 100x.
Sheep antibody to human proNGF: whole serum The sheep anti-proNGF antibodies recognize recombinant proNGF, the prodomain of NGF and but not mouse mature NGF (50 ng in each lane).
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