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Sheep antibody to rh NT4: IgG


Catalogue No. S-059-500
Description FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Batch No. See product label
Unit size 500 ug
Antigen Recombinant human NT4
Other Names Neurotrophin-5; NT-5; Neutrophic factor 5; Neurotrophin-4; NT-4; Neutrophic factor 4; NTF5; NTF4
Accession NT5_HUMAN
Produced in Sheep
Purity Protein G purified IgG
Applications IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a concentration of 2-10 ug/mL for immunohistochemistry, ELISA and Western blot and inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 ug/mL (ED50). Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot.
Cross-reactivity Known to react with NT4 from rat and human, mouse and monkey.
Blast it If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised, Antibody is carrier-free. It contain no BSA or any other protein or sugar added. The material has been lyophilized from simple PBS buffer (10 mM phosphate, 150 mM NaCl, pH 7.2-7.6) without addition of sodium azide or other preservatives.
Reconstitution Reconstitute in 500 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months after purchase
Specific References Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC; Species: Rat
General References 1. Lodouichi (2000) J. Neuroscie. 20(6): 2155
2. Zhang et al. (1999) J Neurosci. Meths. 89 (1): 69
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