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Sheep antibody to SUMO-1 (6-21): whole serum


Catalogue No. S-064-100
Description SUMO-1 binds to a wide range of target proteins as part of a post-translational modification system. Unlike ubiquitin, it does not seem to target protein for degradation, but is involved in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis, apoptosis, protein stability and signal transduction. SUBUNIT: Covalently attached to a number of proteins such as PML, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain. PTM: Cleavage of the last four amino acids of the carboxy-terminus of the precursor form by SENP1 or SENP2 is necessary for function. Several pseudogenes have been reported as well as a number of alternatively spliced isoforms.
Batch No. See product label
Unit size 100 uL
Antigen A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
Other Names Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
Accession SUMO1_HUMAN
Produced in Sheep
Purity Whole serum
Applications IHC, WB. A dilution of 1:2000 to 1:4000 is recommended for immunohistochemistry and 1:4000 to 1:8000 for western blot. Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antiserum recognises human SUMO-1 and not ubiquitin.
Cross-reactivity This antiserum is known to cross react with rat and human SUMO-1.
Blast it If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
References 1. Yang, S.H. et al. Mol. Cell 13(4):611-617 (2004).
2. Ohshima, T. et al. J. Biol. Chem. 278(51):50833-50842 (2003).
3. Bailey, D. et al. J. Biol. Chem. 279(1):692-703 (2004).
4. Ling, Y. et al. Nucleic Acids Res. 32(2):598-610 (2004).
5. Pountney, D.L. et al. Exp. Neurol. 184(1):436-446 (2003).
Images (click to zoom)
Sheep antibody to SUMO-1 (6-21): whole serum Western blot detection of recombinant SUMO-1 protein using Sheep antibody to SUMO-1 (6-21): ws, (catalogue no. S-064-100) at a dilution of 1:8000. The protein sample was 1 µl of E.Coli cell lysate containing approximately 50 ng of recombinant SUMO-1 protein. This antibody recognises a major band of approximately 17 kDa corresponding to SUMO-1, as well as a smaller minor band of 8 kDa.

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