Alpha synuclein, Chicken Polyclonal Antibody

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Catalog Number
C-1795

    Product Info

  • Product Name Alpha synuclein, Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Alpha synuclein Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
  • Alternative Names Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1;
  • Application(s) ICC, IHC-Frozen, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Human, reacts with human, horse, cow, pig, chicken, rat, mouse.
  • Species Reactivity Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
  • Immunogen Description Full length human protein with the epitope from amino acids 61-95
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Alpha synuclein Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
  • Application(s) ICC, IHC-Frozen, WB
  • Application Details Western blotting (1:1,000 - 1:2,000), Immunocytochemistry (1:1,000 - 1:2,000) and Immunohistochemistry (1:1,000 1: 2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Alpha synuclein
  • Specificity Human, reacts with human, horse, cow, pig, chicken, rat, mouse.
  • Target Host Species Human
  • Species Reactivity Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Full length human protein with the epitope from amino acids 61-95
  • Purity Description Affinity purified
  • Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1;
  • Uniprot Number P37840
  • Uniprot Number/Name P37840 (SYUA_HUMAN)
  • Scientific Background May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Ref: uniprot.org.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: Rat cerebellum section stained with chicken anti-α-synuclein antibody (red, 1:3,000) by Immunohistochemistry. Section was co-stained with rabbit anti-GFAP (green, R-1374-50, 1:5,000) and DAPI nuclear dye (blue). IHC method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The α-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells. Right: Western blot analysis of tissue lysates. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord. Antibody dilution: 1:2,000. A strong band at about 15 kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses.

  • Left: Analysis of alpha-synuclein expression in mixed rat neuron-glial cultures by Immunocytochemistry. The alpha-synuclein antibody (red) stains vesicular structures the perikarya and processes of the neurons. The antibody reveals that some of the neuronal perikarya contain more alpha-synuclein than others. Green: MAP2, Blue: DNA. Right: Detection of alpha-synuclein monomer (~15 kDa) in crude rat brain extract by Western Blotting.

    Citations & References