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Small ubiquitin-related modifier 1 (SUMO-1), Rabbit Polyclonal Antibody
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Catalog Number
R-115
- Product Name Small ubiquitin-related modifier 1 (SUMO-1), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Small ubiquitin-related modifier 1 (SUMO-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
- Alternative Names Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
- Application(s) IHC-Frozen, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity This antiserum recognises human SUMO-1 and not ubiquitin. This antiserum is known to cross react with rat and human SUMO-1.
- Species Reactivity Human, Rat
- Immunogen Description A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
- Conjugate Unconjugated
- Purity Description Whole serum
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Small ubiquitin-related modifier 1 (SUMO-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
- Application(s) IHC-Frozen, WB
- Application Details IHC, WB. A dilution of 1:1000 to 1:2000 is recommended for immunohistochemistry and 1:2000 to 1:4000 for western blot. Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Small ubiquitin-related modifier 1 (SUMO-1)
- Specificity This antiserum recognises human SUMO-1 and not ubiquitin. This antiserum is known to cross react with rat and human SUMO-1.
- Target Host Species Human
- Species Reactivity Human, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype Mixed
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
- Purity Description Whole serum
- Format Lyophilized
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
- Uniprot Number P63165
- Uniprot Number/Name P63165 (SUMO1_HUMAN)
- Scientific Background FUNCTION: Ubiquitin-like protein which binds to a wide range of target proteins. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. SUBUNIT: Covalently attached to a number of proteins such as PmL, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
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General References
Yang, S.H. et al. Mol. Cell 13(4):611-617 (2004).
Ohshima, T. et al. J. Biol. Chem. 278(51):50833-50842 (2003).
Bailey, D. et al. J. Biol. Chem. 279(1):692-703 (2004).
Ling, Y. et al. Nucleic Acids Res. 32(2):598-610 (2004).
Pountney, D.L. et al. Exp. Neurol. 184(1):436-446 (2003).