Nicastrin, Central region, Rabbit Polyclonal Antibody

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Catalog Number
R-1685

    Product Info

  • Product Name Nicastrin, Central region, Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Nicastrin, Central region Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Confirmed by WB using peptide absorption.
  • Species Reactivity Human
  • Immunogen Description A synthetic peptide (C-QGETFDYIGSSRMVYD) corresponding to human Nicastrin [331-346] in the central region conjugated via additional N-terminal Cys to Diphtheria toxoid.
  • Conjugate Unconjugated
  • Purity Description Protein G purified IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Nicastrin, Central region Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details WB and IP. Suggested concentration of 3-10 µg/mL is recommended for WB. Human or mouse brain samples commonly prepared with reducing (50 mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Unprocessed full length human Nicastrin is 709 amino acids, however this protein contains an N-terminal signal peptide which is considered to undergo removal/cleavage during processing and transit to the cell plasma membrane, in addition the protein undergoes glycosylation to produce a glycoprotein of about 145 kDa apparent MW by SDS PAGE. Biosensis recommends that the optimal working dilution should be determined by the end user.
  • Target Nicastrin, Central region
  • Specificity Confirmed by WB using peptide absorption.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (C-QGETFDYIGSSRMVYD) corresponding to human Nicastrin [331-346] in the central region conjugated via additional N-terminal Cys to Diphtheria toxoid.
  • Purity Description Protein G purified IgG
  • Format Lyophilized from PBS, pH 7.4. Contains no preservative.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material.
  • Storage Instructions Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Uniprot Number Q92542
  • Uniprot Number/Name Q92542 (NICA_HUMAN)
  • Scientific Background Nicastrin, a type 1 membrane glycoprotein, is an essential component of the gamma secretase complex which is critical for the cleavage of the amyloid precursor protein and other membrane proteins. Nicastrin is widely expressed in different tissue types. This antibody reacts with immature forms of Nicastrin. Detection of higher mol. wt. mature forms is likely to be blocked by glycosylation in this region of the protein.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Immunoblotting with nicastrin central region antibody of mouse Nicastrin protein in PS1-deficient cell line and adult mouse brain (right panel) in comparison with C-terminal Nicastrin antibody (Catalog number R-1684-500). Membrane proteins were prepared and loaded as 20 µg protein per lane.

    Citations & References

  • General References Culvenor, J.G., Ilaya, N.T., Ryan, M.T., Canterford, L., Hoke, D., Williamson, N.A., McLean, C.A., Masters, C.L., and Evin, G. (2004) Characterization of Presenilin complex from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with Presenilin mutations. Eur. J. Biochem. 271, 375-385.
    Ilaya, N.T., Evin, G., Masters, C.L., and Culvenor, J.G. (2004) Nicastrin expression in mouse peripheral tissues is not co-ordinated with Presenilin and is high in muscle. J. Neurochem. 91, 230-23