Ubiquitin C Terminal Hydrolase 1 (UCHL1), Rabbit Polyclonal Antibody

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Catalog Number
R-1408

    Product Info

  • Product Name Ubiquitin C Terminal Hydrolase 1 (UCHL1), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Alternative Names Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1;
  • Application(s) ICC, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, Rat, Ms, Bov, Por
  • Species Reactivity Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
  • Immunogen Description Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Ubiquitin C Terminal Hydrolase 1 (UCHL1)
  • Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, Rat, Ms, Bov, Por
  • Target Host Species Human
  • Species Reactivity Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
  • Purity Description Whole serum
  • Format Lyophilized with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1;
  • Uniprot Number P09936
  • Uniprot Number/Name P09936 (UCHL1_HUMAN)
  • Scientific Background This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Image shows rat mixed neuron/glial cultures stained with Rabbit polyclonal antibody to Ubiquitin C Terminal Hydrolase 1 R-1408-50 (green). Blue is a DNA stain. Note that the Ubiquitin C Terminal Hydrolase 1 (UCHL1) antibody stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also. Surrounding glial cells are not stained with this antibody, though many are present as visualized using the DNA stain.

  • Left: Analysis of SH-SY5Y cells for UCHL1 expression with rabbit antibody to UCHL1 (green, 1:1,000) by Immunocytochemistry. Cells were co-stained with mouse antibody to fibrillarin (M-1372-250, red, 1:1,000). Blue: DAPI nuclear stain. The UCHL1 antibody produces strong staining of the cellular cytoplasm of these cells which share many properties with neurons, while the fibrillarin antibody specifically labels nucleoli. Right: Western blot analysis of tissue and cell lysates using rabbit antibody to UCHL1 (red, 1:2,000, lanes 2-7). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24 kDa corresponds to the UCHL1 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties, but not in HeLa, NIH-3T3 and other non-neuronal cells. The blot was simultaneously probed with a mouse antibody to HSP60 (green).

    Citations & References