Product DescriptiongoogleRabbit anti-Pan-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, FC.
Application(s)FC, WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityThis antibody recognises human, mouse, rat alpha-, beta- and gamma-synuclein.
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide (AKEGVVAAAEKTKQGV) as a consensus part of human alpha-, beta-, and gamma synuclein proteins has been used as the immunogen.
ConjugateUnconjugated
Purity DescriptionPurified by affinity chromatography against the peptide immunogen.
Application DetailsWestern Blotting (0.5-2 μg/mL). Flow Cytometry (10-20 μg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Product ValidationValidated by western blotting and flow cytometry.
TargetPan-synuclein
SpecificityThis antibody recognises human, mouse, rat alpha-, beta- and gamma-synuclein.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (AKEGVVAAAEKTKQGV) as a consensus part of human alpha-, beta-, and gamma synuclein proteins has been used as the immunogen.
Immunogen Length16 amino acids.
EpitopeAmino acids 11-26 of human alpha-synuclein protein.
Purity DescriptionPurified by affinity chromatography against the peptide immunogen.
Physical StateSolid.
FormatLyophilized from PBS, pH 7.2-7.6, containing 0.1% trehalose without preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL of sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for long-term stability. Working aliquots can be kept at 2-8°C for up to 4 weeks, or longer with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundA family of homologous proteins known as alpha-, beta-, and gamma-synuclein are abundantly expressed in brain, especially in the presynaptic terminal of neurons. Although the precise function of these proteins remains unknown, alpha-synuclein has been implicated in synaptic plasticity associated with avian song learning as well as in the pathogenesis of Parkinson's disease (PD), dementia with LBs (DLB), some forms of Alzheimer's disease (AD), and multiple system atrophy (MSA).
Detection of synuclein expression in tissue homogenate and cell lysates by Western Blot. Synuclein monomer is detected at ~14-18 kDa, additional higher-molecular weight bands may represent multimeric forms of synuclein. Method: Reducing SDS-PAGE; Transfer: Towbin’s buffer with 20% methanol; Protein fixation: proteins were fixed on membrane (0.4% formaldehyde, 30 minutes at room temperature) after transfer, as described by Lee & Kamitani, 201; Blocking: 5% skim milk, 1 hour at room temperature; Primary antibody: 1.5 μg/mL, overnight incubation at 2-8°C; Secondary antibody: anti-sheep-HRP (1:6,000); Detection: Chemiluminiscence. Peptide blocking was carried out in presence of two-fold mass excess of peptide over antibody.
Analysis of pan-synuclein expression in rat PC-12 and C6 cell lines by Flow Cytometry. Cells were fixed and permeabilized in methanol (10 minutes). Blocking: 15% horse serum, 30 minutes on ice; Primary antibody: Rabbit polyclonal antibody to pan-synuclein (R-2110-100, red, 20 µg/mL), 60 minutes on ice. Secondary antibody: donkey anti-rabbit-PE (1:100 dilution), 30 minutes in dark on ice. Negative control: Non-specific Control rabbit IgG, (black dashed). Data and results were generated using Orflo MoxiGoTM instrument and protocols.
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