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Neurofilament light polypeptide (NF-L), Mouse Monoclonal Antibody (6H112)

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Catalog Number

M-2114

Product Info

Product Name Neurofilament light polypeptide (NF-L), Mouse Monoclonal Antibody (6H112)
Product Description google Mouse anti-Neurofilament light polypeptide (NF-L), Monoclonal Antibody (Unconjugated), Clone 6H112, suitable for WB and Immunostaining.
Alternative Names NF-L; NF68; NEFL; Neurofilament light polypeptide; Neurofilament protein; Neurofilament triplet L protein; NFL
Application(s) IF, ICC, IHC, WB
Antibody Host Mouse
Antibody Type Monoclonal
Specificity Species cross-reactivity includes human, rat, mouse, cow, and pig.
Species Reactivity Bovine, Human, Mouse, Pig, Rat
Immunogen Description The antibody has been made against the C-terminal peptide of human NF-L protein (amino acids 514-542; peptide GEEEDTKESEEEEKKEESAGEEQVAKKKD) with an N-terminal C added for coupling to KLH.
Conjugate Unconjugated
Purity Description Protein G purified
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-Neurofilament light polypeptide (NF-L), Monoclonal Antibody (Unconjugated), Clone 6H112, suitable for WB and Immunostaining.
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Application(s) IF, ICC, IHC, WB
Application Details Western blot (WB), Immunocytochemistry (ICC) / Immunofluorescence (IF), Immunohistochemistry (IHC). A dilution of 1:5,000 is recommended for WB. A dilution of 1:1000 is recommended for ICC/IF and a dilution of 1:4,000 for IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Neurofilament light polypeptide (NF-L)
Specificity Species cross-reactivity includes human, rat, mouse, cow, and pig.
Target Host Species Human
Species Reactivity Bovine, Human, Mouse, Pig, Rat
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG1
Clone Name 6H112
Conjugate Unconjugated
Immunogen Description The antibody has been made against the C-terminal peptide of human NF-L protein (amino acids 514-542; peptide GEEEDTKESEEEEKKEESAGEEQVAKKKD) with an N-terminal C added for coupling to KLH.
Purity Description Protein G purified
Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
Storage Instructions Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names NF-L; NF68; NEFL; Neurofilament light polypeptide; Neurofilament protein; Neurofilament triplet L protein; NFL
Uniprot Number P07196
Uniprot Number/Name P07196 (NFL_HUMAN)
Scientific Background Neurofilaments are composed of three intermediate filament proteins: light (NF-L ~68 kDa), medium (NF-M ~160 kDa) and heavy (NF-H ~200 kDa), found specifically in neurons, which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins. (Ref: uniprot.org)
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Image of a rat frontal cortex by Immunofluorescence and Immunohistochemistry. The section was stained with M-2114-50, Neurofilament light polypeptide (NF-L), Clone 6H112, Mouse mAb, (green, 1:2000), and co-stained with product R-3771-100, FOX3/NeuN, Rabbit pAb, (red, 1:2,000). Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 µm sections, and free-floating sections were stained. The NF-L antibody labels cell bodies and processes of pyramidal neurons,dendrites, and axons of other neuronal cells. The FOX3/NeuN antibody selectively stains nuclei and cytoplasm of neuronal cells.
Analysis by western blot of NF-L expression in spinal cord lysates from different species with M-2114-50, Neurofilament light polypeptide (NF-L), Clone 6H112, Mouse mAb, (green, 1:5,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: protein standard (red); Lane 2: rat; Lane 3: mouse; Lane 4: pig; Lane 5: cow.

Citations & References