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Neurofilament light polypeptide (NF-L), Mouse Monoclonal Antibody (7D1)

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Catalog Number

M-2112

Product Info

Product Name Neurofilament light polypeptide (NF-L), Mouse Monoclonal Antibody (7D1)
Product Description google Mouse anti-Neurofilament light polypeptide (NF-L), Monoclonal Antibody (Unconjugated), Clone 7D1, suitable for WB, Immunostaining (ICC/IF)
Alternative Names NF-L; NF68; NEFL; Neurofilament light polypeptide; Neurofilament protein; Neurofilament triplet L protein; NFL
Application(s) IF, ICC, WB
Antibody Host Mouse
Antibody Type Monoclonal
Specificity Species cross-reactivity includes human, rat, mouse, cow, pig and horse.
Species Reactivity Bovine, Horse, Human, Mouse, Pig, Rat
Immunogen Description The antibody has been made against a preparation of recombinant full length human NF-L; native protein purified from pig spinal cord. The antibody binds NF-L from a variety of species including human, rat and mouse.
Conjugate Unconjugated
Purity Description Protein G purified
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-Neurofilament light polypeptide (NF-L), Monoclonal Antibody (Unconjugated), Clone 7D1, suitable for WB, Immunostaining (ICC/IF)
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Application(s) IF, ICC, WB
Application Details Western blot (WB), Immunocytochemistry (ICC) / Immunofluorescence (IF). Immunohistochemistry is not recommended. A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for ICC/IF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Neurofilament light polypeptide (NF-L)
Specificity Species cross-reactivity includes human, rat, mouse, cow, pig and horse.
Target Host Species Human
Species Reactivity Bovine, Horse, Human, Mouse, Pig, Rat
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG2b
Clone Name 7D1
Conjugate Unconjugated
Immunogen Description The antibody has been made against a preparation of recombinant full length human NF-L; native protein purified from pig spinal cord. The antibody binds NF-L from a variety of species including human, rat and mouse.
Purity Description Protein G purified
Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
Storage Instructions Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names NF-L; NF68; NEFL; Neurofilament light polypeptide; Neurofilament protein; Neurofilament triplet L protein; NFL
Uniprot Number P07196
Uniprot Number/Name P07196 (NFL_HUMAN)
Scientific Background Neurofilaments are composed of three intermediate filament proteins: light (NF-L ~68 kDa), medium (NF-M ~160 kDa) and heavy (NF-H ~200 kDa), found specifically in neurons, which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.(Ref: uniprot.org)
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Image of a mouse cerebellum by Immunofluorescence. The section was stained with M-2112-50, Neurofilament light polypeptide (NF-L), Clone 7D1, Mouse mAb, (green, 1:5000), and co-stained with product chicken antibody to FOX2, (red, 1:2,000) IF Method: Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 µm sections, and free-floating sections were stained. The NF-L antibody labels dendrites and axons of neuronal cells, predominantly axons in white matter and basket cell axons associated with Purkinje cells. The FOX2 antibody reveals protein expressed in a subset of neurons, including Purkinje cells.
Analysis by western blot of NF-L expression in different tissue lysates with M-2112-50, Neurofilament light polypeptide (NF-L), Clone 7D1, Mouse mAb, (green, 1:5,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: protein standard (red); Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord.

Citations & References