Neurofilament light polypeptide (NF-L), Rabbit Polyclonal Antibody

Image of a mouse cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with R-1392-50, Neurofilament light polypeptide (NF-L), Rabbit pAb, (red, 1:5,000), and co-stained with product C-1383-50, Myelin basic protein, Chicken pAb, (green, 1:5,000). Method: Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The NF-L antibody labels dendrites and axons of neuronal cells, and the MBP antibody stains myelin sheathes around axons.

Analysis by western blot of NF-L expression in different tissue lysates with R-1392-50, Neurofilament light polypeptide (NF-L), Rabbit pAb, (green, 1:20,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: protein standard (red); Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord.

Image of mixed neuron and glial cultures, from a newborn rat brain, stained by Immunofluorescence and Immunocytochemistry. These cultures were stained with R-1392-50, Neurofilament light polypeptide (NF-L), Rabbit pAb, (red) and co stained with product M-1400-500, Peripherin, Clone 7C5, Mouse mAb, (green). A class of large neurons, like the one in the middle of this image, contain Peripherin, while the majority of neurons and their processes contain NF-L and not Peripherin. Interestingly, the Periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA