Product DescriptiongoogleNeurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR.
The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF (S-015-500), NGF (S-052-500), NT3 (S-057-500) and NT4/5 (S-059-500). The kit is completed with one vial of normal sheep IgG (S-1754-100) for use as negative control. This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
Application(s)IHC, Neutralize
Antibody HostSheep
Antibody TypePolyclonal
SpecificityBDNF Antibody: A cross-reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by ELISA. NGF Antibody: A cross-reactivity of less than 1% against recombinant human BDNF, NT3 and NT4/5 by ELISA has been shown. NT3 Antibody: A cross-reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by ELISA. NT4/5 Antibody: Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Neurotrophins share a large degree of homology among human, rodent and other mammalian species, thus these neurotrophin antibodies are useful for a number of species, including human, mouse and rat.
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionBDNF: recombinant human BDNF NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE) NT3: recombinant human NT3 NT4/5: recombinant human NT4
Product DescriptionNeurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR.
The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF (S-015-500), NGF (S-052-500), NT3 (S-057-500) and NT4/5 (S-059-500). The kit is completed with one vial of normal sheep IgG (S-1754-100) for use as negative control. This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
Application DetailsImmunohistochemistry: 1-10 µg/mL. BDNF antibody works best on Zamboni's fixed, frozen tissue, and is not recommended for paraffin-embedded tissue. ELISA: 1-10 µg/mL. Western Blotting: 1-10 µg/mL. Western blotting is not recommended for the BDNF antibody. Inhibition of biological activity in vitro/in vivo: Expected ED50 (in vivo) values are in the range of 2-10 µg/mL. The NGF antibody completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The negative control (normal sheep IgG) should be used at the same concentration as the neurotrophin antibodies.
TargetNeurotrophins
SpecificityBDNF Antibody: A cross-reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by ELISA. NGF Antibody: A cross-reactivity of less than 1% against recombinant human BDNF, NT3 and NT4/5 by ELISA has been shown. NT3 Antibody: A cross-reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by ELISA. NT4/5 Antibody: Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Neurotrophins share a large degree of homology among human, rodent and other mammalian species, thus these neurotrophin antibodies are useful for a number of species, including human, mouse and rat.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostSheep
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionBDNF: recombinant human BDNF NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE) NT3: recombinant human NT3 NT4/5: recombinant human NT4
Purity DescriptionProtein G purified IgG.
FormatLyophilized from PBS, pH 7.4, without preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute each vial with 100 µL sterile-filtered, ultrapure water (KIT-NT-100), or 500 µL sterile-filtered 1X PBS, pH 7.2-7.6 (KIT-NT-500), to achieve an antibody concentration of 1 mg/mL. Centrifuge briefly to remove any insoluble material.
Storage InstructionsStore lyophilized, unopened vials at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundNeurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR.
The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF (S-015-500), NGF (S-052-500), NT3 (S-057-500) and NT4/5 (S-059-500). The kit is completed with one vial of normal sheep IgG (S-1754-100) for use as negative control. This full size version of Biosensis' Neurotrophin Antibody Kit contains 500 μg sheep IgG of each antibody.
This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.