Neurotrophin Antibody Kit, Sheep Polyclonal Antibody

As low as US$297.00
Only %1 left
Catalog Number
KIT-NT

    Product Info

  • Product Name Neurotrophin Antibody Kit, Sheep Polyclonal Antibody
  • Product Description google Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR.

    The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF (S-015-500), NGF (S-052-500), NT3 (S-057-500) and NT4/5 (S-059-500). The kit is completed with one vial of normal sheep IgG (S-1754-100) for use as negative control. This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
  • Application(s) IHC, Neutralize
  • Antibody Host Sheep
  • Antibody Type Polyclonal
  • Specificity BDNF Antibody: A cross-reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by ELISA.
    NGF Antibody: A cross-reactivity of less than 1% against recombinant human BDNF, NT3 and NT4/5 by ELISA has been shown.
    NT3 Antibody: A cross-reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by ELISA.
    NT4/5 Antibody: Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Neurotrophins share a large degree of homology among human, rodent and other mammalian species, thus these neurotrophin antibodies are useful for a number of species, including human, mouse and rat.
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description BDNF: recombinant human BDNF
    NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
    NT3: recombinant human NT3
    NT4/5: recombinant human NT4
  • Conjugate Unconjugated
  • Purity Description Protein G purified IgG.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR.

    The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF (S-015-500), NGF (S-052-500), NT3 (S-057-500) and NT4/5 (S-059-500). The kit is completed with one vial of normal sheep IgG (S-1754-100) for use as negative control. This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
  • Related Products Brain-derived neurotrophic factor (BDNF), Sheep Polyclonal Antibody
    Beta-nerve growth factor (beta-NGF), Sheep Polyclonal Antibody
    Neurotrophin-3 (NT-3), Sheep Polyclonal Antibody
    Neurotrophin-4/5 (NT-4/5), Sheep Polyclonal Antibody
    Normal sheep IgG from non-immunized animal, Sheep Polyclonal Antibody
  • Application(s) IHC, Neutralize
  • Application Details Immunohistochemistry: 1-10 µg/mL. BDNF antibody works best on Zamboni's fixed, frozen tissue, and is not recommended for paraffin-embedded tissue.
    ELISA: 1-10 µg/mL.
    Western Blotting: 1-10 µg/mL. Western blotting is not recommended for the BDNF antibody.
    Inhibition of biological activity in vitro/in vivo: Expected ED50 (in vivo) values are in the range of 2-10 µg/mL. The NGF antibody completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro.
    Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The negative control (normal sheep IgG) should be used at the same concentration as the neurotrophin antibodies.
  • Target Neurotrophins
  • Specificity BDNF Antibody: A cross-reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by ELISA.
    NGF Antibody: A cross-reactivity of less than 1% against recombinant human BDNF, NT3 and NT4/5 by ELISA has been shown.
    NT3 Antibody: A cross-reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by ELISA.
    NT4/5 Antibody: Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Neurotrophins share a large degree of homology among human, rodent and other mammalian species, thus these neurotrophin antibodies are useful for a number of species, including human, mouse and rat.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Sheep
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description BDNF: recombinant human BDNF
    NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
    NT3: recombinant human NT3
    NT4/5: recombinant human NT4
  • Purity Description Protein G purified IgG.
  • Format Lyophilized from PBS, pH 7.4, without preservatives.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute each vial with 100 µL sterile-filtered, ultrapure water (KIT-NT-100), or 500 µL sterile-filtered 1X PBS, pH 7.2-7.6 (KIT-NT-500), to achieve an antibody concentration of 1 mg/mL. Centrifuge briefly to remove any insoluble material.
  • Storage Instructions Store lyophilized, unopened vials at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Uniprot Number P23560; P01139; P20783; P34130
  • Uniprot Number/Name P23560 (BDNF_HUMAN)
    P01139 (NGF_MOUSE)
    P20783 (NTF3_HUMAN)
    P34130 (NTF4_HUMAN)
  • Scientific Background Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR.

    The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF (S-015-500), NGF (S-052-500), NT3 (S-057-500) and NT4/5 (S-059-500). The kit is completed with one vial of normal sheep IgG (S-1754-100) for use as negative control. This full size version of Biosensis' Neurotrophin Antibody Kit contains 500 μg sheep IgG of each antibody.

    This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

    Citations & References