Application DetailsWB, ELISA, ICC. .Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSuperoxide dismutase 1 (SOD1)
SpecificityHuman SOD1
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG2a, Kappa
Clone Name10D5
ConjugateUnconjugated
Immunogen DescriptionA full length recombinant human superoxide dismutase (SOD1) protein with a GST tag has been used as the immunogen.
Purity DescriptionProtein G purified IgG
FormatLiquid. 1x PBS, pH 7.2
Storage InstructionsKeep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSuperoxide dismutase [Cu-Zn]; Superoxide dismutase 1; hSod1; SOD1;
Scientific BackgroundSOD1 binds copper and zinc ions ans is one of two isozymes responsible for destroying free superoxide radicals which are normally produced within the cells and which are toxic to biological systems. SOD1 is a soluble cytoplasmic protein, acting as a homodimer to convert superoxide radicals to molecular oxygen and hydrogen peroxide. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) which is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis.
Left: Western Blot analysis of SOD1 expression in transfected 293T cell line by mouse antibody to human SOD1. A clear band at ~16 kDa is observed in transfected lysate (Lane 1), while SOD1-IR is absent in control lysate. Right: Analysis of SOD1 expression by Immunocytochemistry in HeLa cell with M-1604-100 (10 µg/mL).