Superoxide dismutase 1 (SOD1), Rabbit Polyclonal Antibody

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Catalog Number
R-126

    Product Info

  • Product Name Superoxide dismutase 1 (SOD1), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Superoxide dismutase 1 (SOD1) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Alternative Names Superoxide dismutase [Cu-Zn]; SOD1
  • Application(s) WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Specificity was confirmed by western blot detecting mouse superoxide dismutase (SOD1). This antiserum is known to react with mouse and rat superoxide dismutase (SOD1) protein.
  • Species Reactivity Mouse, Rat
  • Immunogen Description A synthetic peptide (ASGEPVV LSGQIT) as part of mouse superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Superoxide dismutase 1 (SOD1) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Application(s) WB
  • Application Details WB. A dilution of 1:500 to 1:1000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Superoxide dismutase 1 (SOD1)
  • Specificity Specificity was confirmed by western blot detecting mouse superoxide dismutase (SOD1). This antiserum is known to react with mouse and rat superoxide dismutase (SOD1) protein.
  • Target Host Species Mouse
  • Species Reactivity Mouse, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (ASGEPVV LSGQIT) as part of mouse superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
  • Purity Description Whole serum
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Superoxide dismutase [Cu-Zn]; SOD1
  • Uniprot Number P08228
  • Uniprot Number/Name P08228 (SODC_MOUSE)
  • Scientific Background FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot detection of mouse SOD1 in mouse brain tissue extract (lane 1) when using Rabbit antibody to mouse superoxide dismutase (SOD1; 24-36): whole serum (catalogue no. R-126-100) at a dilution of 1:500. The immunoreactivity of this antibody is completely abolished when it is pre-incubated with the immunising SOD1 peptide (lane 2).

    Citations & References

  • Specific References Park KH et al. (2013) Postnatal muscle modification by myogenic factors modulates neuropathology and survival in an ALS mouse model. Nat Commun. 2013;4:2906.
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    Alexander M.D, et al. Ann. Neurol. 52:680-683(2002).
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    1Bartlett, et al. J. Neuroscience Methods. 98(1): 63-7(2000)