Glutathione peroxidase 1 (GPx-1), Rabbit Polyclonal Antibody

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Catalog Number
R-1588

    Product Info

  • Product Name Glutathione peroxidase 1 (GPx-1), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Glutathione peroxidase 1 (GPx-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, ELISA.
  • Alternative Names Glutathione peroxidase 1; GPx-1; GSHPx-1; Cellular glutathione peroxidase; GPX1;
  • Application(s) ELISA, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Human and rat cellular selenium dependent glutathione peroxidase. Human; rat;
  • Species Reactivity Human, Rat
  • Immunogen Description A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human glutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Glutathione peroxidase 1 (GPx-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, ELISA.
  • Application(s) ELISA, IHC-Paraffin-embedded, WB
  • Application Details IHC, WB and ELISA. This antibody works superbly in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine IHC are 1: 200 to 1: 1,000 depending on tissue and detection method. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. For ELISA, a dilution of 1: 1,000 to 1: 4,000 is recommended. This antiserum has extremely high titre, it stains human and rat glial cells intensely and to some extent also stains neurons. Other tissues have not yet been tested. On WB under reducing conditions recognises a 22kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Glutathione peroxidase 1 (GPx-1)
  • Specificity Human and rat cellular selenium dependent glutathione peroxidase. Human; rat;
  • Target Host Species Human
  • Species Reactivity Human, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human glutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.
  • Purity Description Whole serum
  • Format Lyophilized serum with 0.02% thimerosal
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Glutathione peroxidase 1; GPx-1; GSHPx-1; Cellular glutathione peroxidase; GPX1;
  • Uniprot Number P07203
  • Uniprot Number/Name P07203 (GPX1_HUMAN)
  • Scientific Background Glutathione peroxidase 1 has a role in detoxification of hydrogen peroxide and is one of the most important antioxidant enzymes in humans. It exists as a homotetramer which localises to the cytoplasm. It belongs to the glutathione peroxidase family. Glutathione peroxidase 1 is one of few proteins in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. This protein has a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine repeats. The allele with five alanine repeats is significantly associated with breast cancer risk. At least two alternatively spliced isoforms have been identified.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunohistochemical staining of neurons in rat brain tissue. Incubated with Rabbit polyclonal antibody to glutathione peroxidase 1 (R-1588-100) at 1:100. Secondary antibody donkey anti-rabbit conjugated to Cy3 at 1:100.

    Citations & References