SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (NRGVCMSVGFDQYKCDCTRTGFY) corresponding to a region (28-50) from human Cyclooxygenase-2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human COX-2 (precursor) has a predicted length of 604 residues and MW of 69 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat activated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCyclooxygenase-2 (COX-2)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (NRGVCMSVGFDQYKCDCTRTGFY) corresponding to a region (28-50) from human Cyclooxygenase-2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCyclooxygenase (COX) is an important enzyme in the conversion of arachidonic acid to Prostaglandin H2, the precursor of the series-2 prostanoids. At least 3 splice variants have been found; COX-1, COX-2 and COX-3. COX-2 is induced by cytokines and mitogens and is thought to play a role in inflammatory diseases such as rheumatoid arthritis.