SpecificityThis antibody has been shown to be specific for glutathione peroxidase 1 (GPx-1) protein. Human and rat, other species have not yet been tested.
Species ReactivityHuman, Rat
Immunogen DescriptionA synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human lutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.
Application DetailsIHC, WB. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 2000 to 1: 20000 depending on tissue and detection method. For western blotting a dilution range of 1: 10000 to 1: 100000 is recommended. For ELISA, a dilution of 1: 2000 to 1: 20000 is recommended. This antiserum has extremely high titre, it stains human and rat brain microglia intensely and to some extent also stains neurons. Other tissues have not yet been tested. On Western blotting under reducing conditions recognises a 22kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGlutathione peroxidase 1 (GPx-1)
SpecificityThis antibody has been shown to be specific for glutathione peroxidase 1 (GPx-1) protein. Human and rat, other species have not yet been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Antibody HostSheep
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human lutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.
Purity DescriptionWhole serum
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesGSHPx-1; GPx-1; Cellular glutathione peroxidase; GPX1
Scientific BackgroundGlutathione peroxidase 1 has a role in detoxification of hydrogen peroxide and is one of the most important antioxidant enzymes in humans. It exists as a homotetramer which localises to the cytoplasm. It belongs to the glutathione peroxidase family. Glutathione peroxidase 1 is one of few proteins in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. This protein has a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine repeats. The allele with five alanine repeats is significantly associated with breast cancer risk. Two alternatively spliced isoforms have been identified.
Immunofluorescence histochemistry with sheep antibody to glutathione peroxidase 1 (S-072-100). Staining obtained within microglia in the septal region of rat brain and is doubled labeled to show the presence of GFP protein in these same cells following a transfection experiment. Concentration of antibody used was 1:500.
Specific ReferencesKim J-E et al. (2023) Distinct Roles of CK2- and AKT-Mediated NF-κB Phosphorylations in Clasmatodendrosis (Autophagic Astroglial Death) within the Hippocampus of Chronic Epilepsy Rats Antioxidants (Basel). 12(5):1020Application: Rat, IHC (IF) & WB
Kim J-E et al. (2023) EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus Antioxidants (Basel). 12(4):966Application: Rat, IHC (IF) & WB
Kim J-E et al. (2023) Peroxiredoxin 6 Regulates Glutathione Peroxidase 1-Medited Glutamine Synthase Preservation in the Hippocampus of Chronic Epilepsy Rats Antioxidants (Basel). 12(1):156Application: Rat, IHC (IF).
General ReferencesSukenaga Y, et al. Nucleic Acids Res. 15:7178-7178(1987). Ishida K, et al. Nucleic Acids Res. 15:10051-10051(1987). Mullenbach G.T, et al. Nucleic Acids Res. 15:5484-5484(1987). Chada S, et al. Genomics 6:268-271(1990). Moscow J.A, et al. J. Biol. Chem. 267:5949-5958(1992). Forsberg L, et al. Hum. Mutat. 13:294-300(1999). Kote-Jarai Z, et al. Prostate Cancer Prostatic Dis. 5:189-192(2002). Hamanishi T, et al. Diabetes 53:2455-2460(2004). Ichimura Y, et al. J. Urol. 172:728-732(2004). Strausberg R.L, et al. Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). Kim J-E, et al. Antioxidants. 11(4):756(2022).