SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human breast cancer cell line MCF-7, Hela cells, normal and reactive lymphoid tissues were used to test the specificity of this antibody. Human; Rat;
Species ReactivityHuman, Rat
Immunogen DescriptionSynthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41-54 from human Bcl-2 conjugated to thyroglobin.
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Bcl-2 has a predicted length of 239 aa and MW of 26 kDa. A concentration of 0.4-1.0 µg/mL is recommended to detect Bcl-2 in formalin fixed and paraffin embedded tissues as well as formalin/acetone frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetApoptosis regulator Bcl-2
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human breast cancer cell line MCF-7, Hela cells, normal and reactive lymphoid tissues were used to test the specificity of this antibody. Human; Rat;
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NameBL-2
ConjugateUnconjugated
Immunogen DescriptionSynthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41-54 from human Bcl-2 conjugated to thyroglobin.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundApoptosis regulator Bcl-2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. At least 2 isoforms (alpha and beta) are produced by alternate splicing with different C-terminal ends.