Caspase-8 (CASP-8), Rabbit Polyclonal Antibody

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Catalog Number
R-1350

    Product Info

  • Product Name Caspase-8 (CASP-8), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Caspase-8 (CASP-8) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Alternative Names CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH; MCH5;
  • Application(s) WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Immunogen Description A synthetic peptide (DKVYQMKSKPRGYCLIINNH) corresponding to a region (223-242 aa) from human Caspase-8.
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Caspase-8 (CASP-8) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Application(s) WB
  • Application Details Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human Caspase-8 (Isoform 1 precursor) has a predicted length of 479 amino acids and MW of 55 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Caspase-8 (CASP-8)
  • Specificity The specificity of this antibody has been confirmed by WB against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
  • Target Host Species Human
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (DKVYQMKSKPRGYCLIINNH) corresponding to a region (223-242 aa) from human Caspase-8.
  • Sequence DKVYQMKSKPRGYCLIINNH
  • Purity Description Affinity purified
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH; MCH5;
  • Uniprot Number Q14790
  • Uniprot Number/Name Q14790 (CASP8_HUMAN)
  • Scientific Background Caspase-8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. Many alternatively spliced transcript variants encoding different isoforms have been described. (Ref: Entrez Gene). Recent observations in microglia have implicated caspase 8 as a modulator of microglial activation in absence of cell death.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Blot using Rabbit polyclonal antibody to Caspase-8:
    Lane 1: Rat Thymus tissue lysate
    Lane 2: Rat liver tissue lysate
    Lane 3: MCF-7 Whole cell lysate
    Lane 4: Hela Whole cell lysate
    Lane 5: Smmc Whole cell lysate
    Lane 6: Jurkat Whole cell lysate
    Lane 7: Colo320 Whole cell lysate

    Citations & References

  • General References Burguillos M.A. Caspase signalling controls microglia activation and neurotoxicity. Nature. 2011 Apr 21;472(7343):319-24.