Caspase 6 (CASP-6), Rabbit Polyclonal Antibody

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Catalog Number
R-1705

    Product Info

  • Product Name Caspase 6 (CASP-6), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Caspase 6 (CASP-6) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Alternative Names caspase6; CASP6; CASP-6; Mch2; Apoptotic protease Mch-2
  • Application(s) WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Human Caspase 6 protein, no reactivity to other human caspases Potential reactivity to mouse Caspase 6 based upon sequence homology but as yet untested.
  • Species Reactivity Human
  • Immunogen Description A synthetic peptide corresponding to an amino acid sequence at the N-terminal of human Caspase 6 comprising amino acids 24-44 of human Caspase 6
  • Conjugate Unconjugated
  • Purity Description Affinity purified polyclonal IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Caspase 6 (CASP-6) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Application(s) WB
  • Application Details Western Blot: 0.1ug-0.5 µg/mL cell lysates, ECL detection
    Immunohistochemistry, paraffin embedded: 0.8-2.0 µg/mL with HEIR antigen recovery
    Immunohistochemistry, frozen sections: 0.5-1.0 µg/mL PLP fixation recommended.
  • Target Caspase 6 (CASP-6)
  • Specificity Human Caspase 6 protein, no reactivity to other human caspases Potential reactivity to mouse Caspase 6 based upon sequence homology but as yet untested.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide corresponding to an amino acid sequence at the N-terminal of human Caspase 6 comprising amino acids 24-44 of human Caspase 6
  • Sequence AFYKREMFDPAEKYKMDHRRR
  • Purity Description Affinity purified polyclonal IgG
  • Format Freeze-dried powder from liquid containing PBS, 5 mg BSA, 0.05 mg Thimerosal and 0.05 mg sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute vial in 0.2 mL sterile-filtered, ultrapure water. This will produce a final concentration of 500 µg/mL of antibody. Centrifuge to remove any insoluble material.
  • Storage Instructions Store dry, unopened vial at -20°C for one year; after reconstitution aliquot in to single use sizes and store at 2-8°C for up to one month; store aliquots frozen at -70°C for up to six months, protected from light and prevent multiple freeze thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names caspase6; CASP6; CASP-6; Mch2; Apoptotic protease Mch-2
  • Uniprot Number P55212
  • Uniprot Number/Name P55212 (CASP6_HUMAN)
  • Scientific Background Caspases play several key roles in cellular growth and development, extracellular matrix remodeling, wound healing, homeostasis, and are implicated in a wide range of diseases from auto immune dysfunction to cancer metastases as well. Caspases are Cysteine Aspartate Proteases which form a family of metalloproteinases of which there are more than a dozen members. Caspases are all normally expressed in cells as inactive precursor zymogens that then get activated via proteolytic cleavage to form active enzyme complexes. Activation can occur by several means and programmed cell death, or apoptosis, is among the most studied, but there are several others since caspases are involved in so many critical and disease states. Among the 14 or so members, Caspase-6 is particularly interesting because it has been implicated in playing a role in some neurodegenerative diseases including Alzheimer's and Huntington's disease. Caspase-6 has been shown to cut amyloid precursor protein (APP), at position 720 leading to the toxic fragment Jcasp, which is one of the fragments found in amyloid plaques which are believed to be an indicator of the disease, and in Huntington's disease, the specific amino acid sequence (IVLD586G) is recognized by Caspase-6 on the Huntington protein (htt) in mice with Huntington's disease. The proteolytic cleavage of htt liberates toxic fragments containing the expanded polyglutamine tract that are neurotoxic and that stimulates additional proteolytic activity leading to apoptosis and neurodegeneration. Mutation of the Caspase-6 site in mice model with Alzheimer's and Huntington's disease provides protection from the neural dysfunction, suggesting a causal relationship between Caspase-6 cleavage and neurodegeneration. Biosensis is pleased to offer a rabbit polyclonal antibody to human Caspase 6 that reacts in paraffin embedded immunohistochemistry and western blotting for your continued research into Caspase 6 and its potential roles in both normal and pathological conditions.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

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