Fas ligand (FasL), Rabbit Polyclonal Antibody

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Catalog Number
R-1822
Discontinued

    Product Info

  • Product Name Fas ligand (FasL), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Fas ligand (FasL) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Alternative Names CD95L; CD178 antigen; FASLG; TNFSF6; APT1LG1;
  • Application(s) WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB.
  • Species Reactivity Human
  • Immunogen Description A synthetic peptide (ELSLVNFEESQTFFGLYKL) corresponding to a region (263-281) from human FAS ligand (Fas-L).
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Fas ligand (FasL) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Application(s) WB
  • Application Details Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended. Human Fas-L has a predicted length of 281 residues and a band size between 31 - 40 kDa with glycosylation. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Fas ligand (FasL)
  • Specificity The specificity of this antibody has been confirmed by WB.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (ELSLVNFEESQTFFGLYKL) corresponding to a region (263-281) from human FAS ligand (Fas-L).
  • Sequence ELSLVNFEESQTFFGLYKL
  • Purity Description Affinity purified
  • Format lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names CD95L; CD178 antigen; FASLG; TNFSF6; APT1LG1;
  • Uniprot Number P48023
  • Uniprot Number/Name P48023 (TNFL6_HUMAN)
  • Scientific Background The FAS ligand (Fas-L) belongs to the tumor necrosis factor family. The full length Fas-L protein has a cytoplasmic domain, transmembrane (Type II) domain and extracellular domain. The membrane-bound form of Fas-L is proteolytically cleaved to produce soluble Fas-L. There are at least 2 known isoforms of Fas-L produced by alternate splicing.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot analysis of Fas ligand expression in JURKAT (1) and HL60 (2) cell lysates. Proteins were loaded at 40 ug per lane, primary antibody was used at 0.5 µg/mL.

    Citations & References