Product NameBcl-2-like protein 1 (Bcl2-L-1), Rabbit Polyclonal Antibody
Product DescriptiongoogleRabbit anti-Bcl-2-like protein 1 (Bcl2-L-1) Polyclonal Antibody (Unconjugated), suitable for WB.
Alternative NamesBcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; BCL2L1; BCLX; bcl-xS; bcl-x beta;
Application(s)WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (YQSFEQDTFVE) corresponding to a region (120-130) from human Bcl-2-like protein 1 isoform Bcl-X(S). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Product DescriptionRabbit anti-Bcl-2-like protein 1 (Bcl2-L-1) Polyclonal Antibody (Unconjugated), suitable for WB.
Application(s)WB
Application DetailsWestern Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human Bcl-2-like protein 1 isoform Bcl-X(S) has a predicted length of 170 amino acids and MW of 19 kDa. A concentration of 2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetBcl-2-like protein 1 (Bcl2-L-1)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (YQSFEQDTFVE) corresponding to a region (120-130) from human Bcl-2-like protein 1 isoform Bcl-X(S). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesBcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; BCL2L1; BCLX; bcl-xS; bcl-x beta;
Scientific BackgroundBcl-2-like protein 1 is a single-pass membrane protein belonging to the Bcl-2 family of proteins that can act as anti- or pro-apoptotic regulators. At least 3 isoforms of Bcl-2-like protein 1 are produced by alternative splicing. Isoform Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover such as developing lymphocytes and is an activator of apoptosis.