SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (QEKCRWLASSKGKQVRNFS) corresponding to a region (283-301) from the C-terminus of human XIAP-associated factor 1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human XIAP-associated factor 1 (isoform 1) has a predicted length of 301 residues and MW of 35 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetXIAP-associated factor 1
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (QEKCRWLASSKGKQVRNFS) corresponding to a region (283-301) from the C-terminus of human XIAP-associated factor 1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesBIRC4-binding protein; XAF1; BIRC4BP; XIAPAF1;
Scientific BackgroundXIAP-associated factor 1 (XAF1) binds to X-linked inhibitor of apoptosis (XIAP) and antagonizes the anticaspase activity of XIAP. XAF1 is expressed in all adult and fetal tissues but is frequently down-regulated in cancer cell lines. At least 7 isoforms of XAF1 are produced by alternative splicing.