Autophagy-related protein 12 (ATG12), Mouse Monoclonal Antibody

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Catalog Number
M-879

    Product Info

  • Product Name Autophagy-related protein 12 (ATG12), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Autophagy-related protein 12 (ATG12) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names Autophagy-related protein 12; ATG12; APG12; APG12L; APG12-like;
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Species Reactivity Human
  • Immunogen Description Full recombinant human ATG12, isoform 2, (1-74) with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Autophagy-related protein 12 (ATG12) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Autophagy-related protein 12 (ATG12)
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 2H8
  • Conjugate Unconjugated
  • Immunogen Description Full recombinant human ATG12, isoform 2, (1-74) with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.2
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Autophagy-related protein 12; ATG12; APG12; APG12L; APG12-like;
  • Uniprot Number O94817
  • Uniprot Number/Name O94817 (ATG12_HUMAN)
  • Scientific Background
    Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy. ATG12 is conjugated to ATG5. The ATG12-ATG5 conjugate has an apparent molecular mass of 65 kDa. The C-terminal glycine of ATG12 is conjugated to a central lysine (lys130) of ATG5. Studies showed that over expression of ATG3 facilitated formation of the ATG12-ATG5 conjugate, suggesting that ATG3 cross-talks with the ATG12 conjugation system. ATG12 is ubiquitously expressed.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot detection of GST tagged recombinant human ATG12. Note the GST tag alone is 26 kDa.

  • Detection limit of 1 ng/mL for GST tagged recombinant ATG12 when the anti-ATG12 primary antibody, catalogue number M-879-100, is used as a capture antibody in sandwich ELISA.

    Citations & References

  • Specific References De la Mata M. et al (2015) Pharmacological Chaperones and Coenzyme Q10 Treatment Improves Mutant _-Glucocerebrosidase Activity and Mitochondrial Function in Neuronopathic Forms of Gaucher Disease. Sci Rep. 2015 Jun 5;5:10903.

    Garrido-Maraver J. et al (2015) Critical role of AMP-activated protein kinase in the balance between mitophagy and mitochondrial biogenesis in MELAS disease. Biochim Biophys Acta. 2015 Nov;1852(11):2535-53.

    Garrido-Maraver J. et al (2012) Screening of effective pharmacological treatments for MELAS syndrome using yeasts, fibroblasts and cybrids models of the disease. Br J Pharmacol. 2012 Jul 2.

    De la Mata M. et al (2012) Recovery of MERRF fibroblasts and cybrids pathophysiology by Coenzyme Q₁₀. Neurotherapeutics. 2012 Apr;9(2):446-63.

    Bullon P et al (2012) Autophagy in periodontitis patients and gingival fibroblasts: unraveling the link between chronic diseases and inflammation. BMC Med. 2012 Oct 17;10:122.

    Cotan D et al (2011) Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts. FASEB J. 2011 Aug;25(8):2669-87.

    Rodriguez-Hernandez A et al (2009) Coenzyme Q deficiency triggers mitochondria degradation by mitophagy. Autophagy. 2009 Jan;5(1):19-32.