Product DescriptionMouse anti-Autophagy-related protein 12 (ATG12) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAutophagy-related protein 12 (ATG12)
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name2H8
ConjugateUnconjugated
Immunogen DescriptionFull recombinant human ATG12, isoform 2, (1-74) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAutophagy-related protein 12; ATG12; APG12; APG12L; APG12-like;
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy. ATG12 is conjugated to ATG5. The ATG12-ATG5 conjugate has an apparent molecular mass of 65 kDa. The C-terminal glycine of ATG12 is conjugated to a central lysine (lys130) of ATG5. Studies showed that over expression of ATG3 facilitated formation of the ATG12-ATG5 conjugate, suggesting that ATG3 cross-talks with the ATG12 conjugation system. ATG12 is ubiquitously expressed.
Western blot detection of GST tagged recombinant human ATG12. Note the GST tag alone is 26 kDa.
Detection limit of 1 ng/mL for GST tagged recombinant ATG12 when the anti-ATG12 primary antibody, catalogue number M-879-100, is used as a capture antibody in sandwich ELISA.
Specific ReferencesDe la Mata M. et al (2015) Pharmacological Chaperones and Coenzyme Q10 Treatment Improves Mutant _-Glucocerebrosidase Activity and Mitochondrial Function in Neuronopathic Forms of Gaucher Disease. Sci Rep. 2015 Jun 5;5:10903.
Garrido-Maraver J. et al (2015) Critical role of AMP-activated protein kinase in the balance between mitophagy and mitochondrial biogenesis in MELAS disease. Biochim Biophys Acta. 2015 Nov;1852(11):2535-53.
Garrido-Maraver J. et al (2012) Screening of effective pharmacological treatments for MELAS syndrome using yeasts, fibroblasts and cybrids models of the disease. Br J Pharmacol. 2012 Jul 2.
Bullon P et al (2012) Autophagy in periodontitis patients and gingival fibroblasts: unraveling the link between chronic diseases and inflammation. BMC Med. 2012 Oct 17;10:122.
Cotan D et al (2011) Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts. FASEB J. 2011 Aug;25(8):2669-87.
Rodriguez-Hernandez A et al (2009) Coenzyme Q deficiency triggers mitochondria degradation by mitophagy. Autophagy. 2009 Jan;5(1):19-32.