Product DescriptionMouse anti-Autophagy-related protein 3 (ATG3) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB, sandwich ELISA and Flow Cytometry (2μg/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAutophagy-related protein 3 (ATG3)
SpecificitySpecificity has been confirmed by WB and ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG2b, kappa
Clone Name1F7
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human ATG3 [1-100] with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAutophagy-related protein 3; APG3-like; hApg3; Protein PC3-96; ATG3; APG3; APG3L
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. ATG3 is an E2-like protein-conjugating enzyme for GABARAPL1, GABARAPL2 and MAPLC3 binding them to PE. ATG3 forms an E1-E2 complex with ATG7 and cross-talks with the ATG12 conjugation system. ATG3 is a cytoplasmic protein that is widely expressed with highest expression in heart, skeletal muscle, kidney , liver and placenta.
A: Flow Cytometry analysis of ATG3 expression in SH-SY5Y cells. Fixing and permeabilization of cells: absolute methanol and 0.1% Tween-20 in PBS; Primary antibody: Mouse Monoclonal antibody to ATG3 (cat # M-908-100, 2 μg per ~106 cells) for 30 minutes at room temperature; Secondary antibody: Goat anti-mouse PE (1:100 dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions. Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Western blot detection of ATG3 expression in Jurkat cell lysate. C: Sandwich ELISA using the anti-ATG3 monoclonal antibody as capture antibody. The detection limit for GST-tagged recombinant ATG3 is 1 ng/mL.
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