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Autophagy-related protein 5 (ATG5), Mouse Monoclonal Antibody
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Catalog Number
M-881
- Product Name Autophagy-related protein 5 (ATG5), Mouse Monoclonal Antibody
- Product Description Mouse anti-Autophagy-related protein 5 (ATG5) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
- Alternative Names Autophagy protein 5; APG5-like; Apoptosis -specific protein; ATG5; APG5L; ASP; APG5
- Application(s) ELISA, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
- Species Reactivity Human
- Immunogen Description Partial recombinant human ATG5 (176-275) with a GST tag.
- Conjugate Unconjugated
- Purity Description Protein G purified immunoglobulin
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Autophagy-related protein 5 (ATG5) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
- Application(s) ELISA, WB
- Application Details This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Autophagy-related protein 5 (ATG5)
- Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
- Target Host Species Human
- Species Reactivity Human
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG
- Clone Name 3D2
- Conjugate Unconjugated
- Immunogen Description Partial recombinant human ATG5 (176-275) with a GST tag.
- Purity Description Protein G purified immunoglobulin
- Format Lyophilized from PBS pH 7.2
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Autophagy protein 5; APG5-like; Apoptosis -specific protein; ATG5; APG5L; ASP; APG5
- Uniprot Number Q9H1Y0
- Uniprot Number/Name Q9H1Y0 (ATG5_HUMAN)
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Scientific Background
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG5 conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. It localise to the cytoplasm and it co-localise with nonmuscle actin. ATG5 is ubiquitously expressed. Its expression is dramatically increased in apoptotic cells. Conjugation to ATG12 is essential for autophage but it is not required for association with isolation membrane.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
